Skip to Content
Merck
All Photos(1)

Documents

Safety Information

76546

Sigma-Aldrich

Lipase, immobilized on Immobead 150 from Thermomyces lanuginosus

≥3000 U/g

Synonym(s):

Immobilized lipase, Lipase enzyme

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352204
NACRES:
NA.54

biological source

fungus (Thermomyces lanuginosus)

form

powder (or beads)

specific activity

≥3000 U/g

technique(s)

analytical sample preparation: suitable

color

white to off-white

application(s)

life science and biopharma

storage temp.

2-8°C

General description

Research area: Cell Signaling

Lipase from Thermomyces lanuginosus (TLL) is a basophilic, single-chain protein and thermostable enzyme. It belongs to the alpha/beta-hydrolase fold family. TLL is roughly spherical and is made up of a central seven-stranded β-parallel sheet with five interconnecting α-helices.

Application

Lipase, immobilized on Immobead 150 from Thermomyces lanuginosus has been used:
  • as a biocatalyst to study ultrasound-assisted transesterification of a blend of non-edible oils with methanol.
  • as a catalyst in the synthesis of medium chain fatty acids (MCFA) and polyunsaturated fatty acids (PUFA) rich oils by enzymatic structuring of flax seed oil.
  • for the resolution of chiral compounds and the transesterification production of biodiesel.
  • to enhance the efficiency and selectivity of the enzymatic synthesis of ethyl-(R)-2-hydroxy-4-phenylbutyrate, (R)-HPB ester.
  • to facilitate a chemoenzymatic process for the continuous synthesis of (S)-7-((tert-Butyldiphenylsilyl)oxy)hept-1-yn-4-ol, a crucial intermediate for Eribulin synthesis, within a packed-bed reactor setup.

Biochem/physiol Actions

It participates in catalyzing the hydrolysis of triglycerides at the water-lipid interface and produces mono-, di-glycerides, and free fatty acids. Due to its versatility, this enzyme is applicable in various industries including, detergents, paper, leather, baking, fine chemicals, fuel, and biocatalysts.
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol butyric acid per minute at pH 7.5 and 40°C (tributyrin, Cat. No. 91010, as substrate)

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

76546-BULK:
76546-10G:
76546-VAR:
76546-50G:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Study of Thermomyces lanuginosa Lipase in the Presence of Tributyrylglycerol and Water
Santini S, et al.
Biophysical Journal, 96(12), 4814?4825-4814?4825 (2009)
Chemoenzymatic Process for the Preparation of (S)-7-((tert-Butyldiphenylsilyl)oxy)hept-1-yn-4-ol in a Continuous Packed-Bed Reactor, a Key Intermediate for Eribulin Synthesis
Krishna AS, et al.
Organic Process Research & Development, 24(11), 2657?2664-2657?2664 (2020)
An improved process for the preparation of ethyl-(R)-2-hydroxy-4-phenylbutyrate, (R)-HPB ester by lipase from Thermomyces lanuginosus
Basetty S, et al.
bioresource technology reports, 101163-101163 (2022)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service