72559

Nitrilotriacetic acid

BioUltra, ≥99.0% (T)

• Synonym(s): N,N-Bis(carboxymethyl)glycine, NTA, Tris(carboxymethyl)amine
• Linear Formula: N(CH₂COOH)₃
• CAS Number: 139-13-9
• Molecular Weight: 191.14
• Beilstein: 1710776
• EC Number: 205-355-7
• MDL number: MFCD00004287
• UNSPSC Code: 12352102
• PubChem Substance ID: 57652424
• NACRES: NA.25

Properties

• product line: BioUltra
• Quality Level: 200
• Assay: ≥99.0% (T)
• form: powder
• reaction suitability: reagent type: reductant
• impurities: insoluble matter, passes filter test
• ign. residue: ≤0.05%
• loss: ≤0.1% loss on drying, 110 °C
• pH: 1.7-2.7 (23 °C, 10 g/L)
• mp: 245 °C (dec.) (lit.)
• solubility: 0.1 M NaOH: 0.1 M at 20 °C, clear, colorless
• anion traces: chloride (Cl^-): ≤100 mg/kg
• cation traces: Al: ≤20 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤20 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤500 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 0.1 M in 1 M NaOH
• UV absorption: λ: 260 nm A_max: 1.5; λ: 280 nm A_max: 0.05
• SMILES string: OC(=O)CN(CC(O)=O)CC(O)=O
• InChI: 1S/C6H9NO6/c8-4(9)1-7(2-5(10)11)3-6(12)13/h1-3H2,(H,8,9)(H,10,11)(H,12,13)
• InChI key: MGFYIUFZLHCRTH-UHFFFAOYSA-N

Description

Application

Nitrilotriacetic has been used in the preparation of nitrilotriacetic (NTA) solution for Ni-NTA slide generation.

An excellent chelating agent.

Biochem/physiol Actions

Nitrilotriacetic (NTA) has the ability to link with histidine side chain of proteins. It can be used in fluorescent labelling, hexahistidine (His6)-tagged proteins for purification and surface immobilization.

Other Notes

Chelating agent; As Pb²⁺ buffer, preparation and properties; In a mixture of Ca²⁺-buffering ligands; Encapsulation of radioactive metal ions in liposomes; Compilation and critical evaluation by IUPAC of published data on stability constants of NTA complexes in solution.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Warning
• Hazard Statements: H302,H319,H351
• Precautionary Statements: P202 - P264 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): 212.0 °F - closed cup
• Flash Point(C): 100 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves