66540780

STBCi058-C

Human iPS Cell Line

• UNSPSC Code: 41106509

Properties

• biological source: human dermis (fibroblast)
• reprogramming method: Sendai virus
• description: age (N/A)
• manufacturer/tradename: EBiSC^™
• gender: male
• growth mode: adherent (pluripotent)
• technique(s): cell culture | stem cell: suitable
• relevant disease(s): diabetes (mellitus)
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Cell Line Origin

Depositor
StemBANCC

Cell Line Description

Derivation
Primary cell type: Fibroblast
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Is the reprogramming vector detectable: Unknown

Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no

Characterization
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative

Genotyping
Karyotyping
Cell line karyotype: No abnormalities detected
Karyotyping method: Molecular karyotyping by SNP array

Linkage

• Additional Cell Line Info
• EBiSC Access Use Agreement (EAUA)
• Cell Line Information Pack (CLIP)

Note: EAUA and CLIP must be completed before order fulfillment

Subculture Routine

Surface coating: Matrigel^®/Geltrex^®
Passage method: Enzyme-free cell dissociation / EDTA
O₂ concentration: 21%
CO₂ concentration: 5%
Culture medium: mTeSR^® 1

Legal Information

EBiSC is a trademark of Fraunhofer-Gesellschaft

GELTREX is a registered trademark of Life Technologies Corporation

Matrigel is a registered trademark of Corning, Inc.

mTeSR is a registered trademark of WiCell Research Institute, Inc.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable