66540604

BIONi037-A-2

Human iPS Cell Line

• UNSPSC Code: 41106509

Properties

• biological source: human
• reprogramming method: episomal
• description: age (N/A)
• manufacturer/tradename: EBiSC^™
• gender: female
• growth mode: adherent (pluripotent)
• technique(s): cell culture | stem cell: suitable
• relevant disease(s): Alzheimer′s disease
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Cell Line Origin

Depositor
Bioneer

Cell Line Description

Derivation
Primary cell type: -
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr

Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no

Characterization
Analysis of Undifferentiated Cells
Morphology:
03-08-2017 16423 apoe 22.tif
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative

Genotyping
Karyotyping
Passage number: 23
Cell line karyotype: 46 XY
Karyotyping method: G-Banding
Image / Result

Genetic Modification
Disease/phenotype related modifications
Disease: Alzheimer′s disease
Type of modification: Isogenic
Chromosome location: 19q13.32
Nucleotide sequence HGSV: rs7412 (T/T); rs429358 (T/T)
Protein sequence HGSV: 112Cys, 158Cys
Zygosity: Homozygous
Target locus modification: The bases at both SNPs rs7412 and rs429358 were changed to a T

Linkage

• Additional Cell Line Info
• EBiSC Access Use Agreement (EAUA)
• Cell Line Information Pack (CLIP)

Note: EAUA and CLIP must be completed before order fulfillment

Subculture Routine

Surface coating: Matrigel^®/Geltrex^®
Culture medium: Essential E8^®

Legal Information

E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation

EBiSC is a trademark of Fraunhofer-Gesellschaft

GELTREX is a registered trademark of Life Technologies Corporation

Matrigel is a registered trademark of Corning, Inc.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable