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Safety Information

64010

Sigma-Aldrich

Metanil Yellow

for microscopy (Hist.), indicator (pH 1.2-2.3)

Synonym(s):

3-(4-Anilinophenylazo)benzenesulfonic acid sodium salt, Acid Yellow 36

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About This Item

Empirical Formula (Hill Notation):
C18H14N3NaO3S
CAS Number:
587-98-4
Molecular Weight:
375.38
Colour Index Number:
13065
Beilstein:
3831568
EC Number:
209-608-2
MDL number:
MFCD00007486
UNSPSC Code:
12171500
PubChem Substance ID:
329759919
NACRES:
NA.47

grade

for microscopy (Hist.)
indicator (pH 1.2-2.3)

form

powder

solubility

methanol: 0.01 g/10 mL, orange to very deep orange

εmax

≥500 at 409-419  nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[O-]S(=O)(=O)c1cccc(c1)\N=N\c2ccc(Nc3ccccc3)cc2

InChI

1S/C18H15N3O3S.Na/c22-25(23,24)18-8-4-7-17(13-18)21-20-16-11-9-15(10-12-16)19-14-5-2-1-3-6-14;/h1-13,19H,(H,22,23,24);/q;+1/p-1/b21-20+;

InChI key

NYGZLYXAPMMJTE-ANVLNOONSA-M

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Application

Metanil yellow has been used for staining of leukocyte granules.[1] It is also used as a counterstain in microscopic sections.[2]

Biochem/physiol Actions

Metanil yellow is an azo dye.[3] It is used as a counterstain in PAS (periodic acid-Schiff)/iron-hematoxylin stained sections to increase contrast.[4]

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H318,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

64010-100G-F:
64010-VAR-F:
64010-25G-F:
64010-BULK-F:

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Certificates of Analysis (COA)

Lot/Batch Number
BCCD4503
BCCC0847
BCCB6873
BCBQ7911V
BCBP5613V

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An improved stain for connective tissue cells in plastic-embedded sections.
Laboratory Medicine, 10, 783-785 (1979)
Enhancement of histological detail using metanil yellow as counterstain in periodic acid Schiff's hematoxylin staining of glycol methacrylate tissue sections.
Quintero-Hunter I, et al.
Biotechnic & Histochemistry, 66, 169-169 (1991)
Photocatalytic degradation of azo dye Metanil Yellow: optimization and kinetic modeling using a chemometric approach.
Applied Catalysis. B, Environmental, 77, 1-11 (2007)
O Anjaneya et al.
Journal of hazardous materials, 190(1-3), 351-358 (2011-04-08)
Two different bacterial strains capable of decolorizing a highly water soluble azo dye Metanil Yellow were isolated from dye contaminated soil sample collected from Atul Dyeing Industry, Bellary, India. The individual bacterial strains Bacillus sp. AK1 and Lysinibacillus sp. AK2
M Das et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 35(8), 835-838 (1997-08-01)
Metanil yellow, a non-permitted food colour, has been found in various foodstuffs. The induction potential of metanil yellow on hepatic microsomal cytochrome P-450 (P-450)-dependent monooxygenases and cytosolic detoxification enzymes, namely, glutathione S-transferase (GST) and quinone reductase (QR), was investigated. Oral
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