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60142

Sigma-Aldrich

Potassium chloride solution

BioUltra, for molecular biology, ~1 M in H2O

Synonym(s):

KCl, potassium chloride

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100 ML
¥11,100
500 ML
¥52,100

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100 ML
¥11,100
500 ML
¥52,100

About This Item

Linear Formula:
KCl
CAS Number:
Molecular Weight:
74.55
Beilstein:
1711999
MDL number:
UNSPSC Code:
12352302
PubChem Substance ID:
NACRES:
NA.26
grade:
for molecular biology

¥11,100

List Price¥15,800Save 30%

In StockDetails


Request a Bulk Order

grade

for molecular biology

Quality Level

product line

BioUltra

concentration

~1 M in H2O

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.001% total nitrogen (N)

pH

5.0-8.0 (25 °C, 1 M in H2O)

density

1.04 g/mL at 20 °C

anion traces

sulfate (SO42-): ≤10 mg/kg

cation traces

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Na: ≤20 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: 0.02
λ: 280 nm Amax: 0.01

SMILES string

[Cl-].[K+]

InChI

1S/ClH.K/h1H;/q;+1/p-1

InChI key

WCUXLLCKKVVCTQ-UHFFFAOYSA-M

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General description

Potassium is an intracellular cation with just 2% of total body K+ inhabiting the extracellular fluid. The distribution of K+ between the intracellular and extracellular space is analyzed by the action of several transport pathways like Na+-K+-ATPase, the Na+-K+-2Cl-cotransporter NKCC1, the four KCCs, and a plethora of K+ channels. Also, skeletal muscle comprises almost 75% of total body potassium and elicits considerable influence on extracellular K+.[1] Potassium chloride is consumed orally to prevent or correct potassium depletion caused by diuretic use or other conditions. It is used as a food supplement and salt substitute.[2]

Application

Potassium chloride solution has been used as a component of:
  • multiple annealing and looping based amplification cycles (MALBAC) lysis buffer to digest proteins for single-cell multiomics sequencing[3]
  • single-cell tagged reverse transcription (STRT) buffer, and STRT-RT buffer for single-cell RNA-sequencing[4]
  • phosphate buffer saline (PBS) to wash transfected cells[5]

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

60142-100ML-F:
60142-BULK-F:
60142-VAR-F:
60142-500ML-F:


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Certificates of Analysis (COA)

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Meyler's Side Effects of Drugs (2016)
Transport of Sodium, Chloride, and Potassium
Brenner and Rector's The Kidney E-book (2019)
Takanobu Kato et al.
Nature protocols, 1(5), 2334-2339 (2007-04-05)
Hepatitis C virus (HCV) infection causes chronic liver disease and is a worldwide health problem. Despite ever-increasing demand for knowledge on viral replication and pathogenesis, detailed analysis has been hampered by a lack of efficient viral culture systems. We isolated
Yuanyuan Qu et al.
Nucleic acids research, 41(10), 5263-5272 (2013-04-13)
Bacterial nucleoid-associated proteins, such as H-NS-like proteins in Enterobacteriaceae, are abundant DNA-binding proteins that function in chromosomal DNA organization and gene transcription regulation. The Mycobacterium tuberculosis Lsr2 protein has been proposed to be the first identified H-NS analogue in Gram-positive
Calista K L Ng et al.
Human molecular genetics, 24(11), 3163-3171 (2015-02-26)
mRNA decay is an essential and active process that allows cells to continuously adapt gene expression to internal and environmental cues. There are two mRNA degradation pathways: 3' to 5' and 5' to 3'. The DCPS protein is the scavenger

Questions

  1. Why is it approximately (~) 1 M? What is the tolerance in concentration around 1 M?

    1 answer
    1. The certificate of analysis for this product (a sample of which can be seen in the 'Documentation' section of the product page) indicates that the specification range is 0.95 to 1.10 M.

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