5008

Human Collagen Type I & III

from neo-natal fibroblast cells, 97% Type I with the remainder of Type III collagen, powder, suitable for cell culture, VitroCol^®

• Synonym(s): Collagen Solution
• EC Number: 232-697-4
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• product name: VitroCol^®, Collagen from Human neonatal fibroblasts, 97% Type I with the remainder of Type III collagen, suitable for cell culture
• Quality Level: 100
• sterility: sterile-filtered
• form: lyophilized
• packaging: pkg of 15 mg
• manufacturer/tradename: Advanced BioMatrix
• technique(s): cell culture | mammalian: suitable
• impurities: <1 EU/mL Endotoxin level
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

VitroCol^® collagen is naturally secreted from human neo-natal fibroblast cells. The human fibroblasts are cultured in optimal conditions allowing the fibroblasts to naturally and efficiently secret extracellular matrix. The extracellular matrix is then processed and purified to yield the naturally produced human collagen.

VitroCol^® is approximately 97% Type I human collagen with the remainder being comprised of Type III collagen. It contains high monomer content as measured by gel permeation chromatography.

VitroCol^® is especially ideal for human cell culture systems when coating of surfaces and providing preparations of thin layers of culturing cells. It is not recommend for the formation of a solid gel. VitroCol^® is supplied as a sterile, lyophilized powder.

Preparation Note

This product is supplied as a lyophilized powder with 15 mg of human collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

Legal Information

VitroCol is a registered trademark of Advanced BioMatrix, Inc.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable