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3514-05A

Sigma-Aldrich

Human Carotid Artery Smooth Muscle Cells: HCtASMC, adult

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

human carotid artery (normal)

Quality Level

100

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

karyotype

23

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

stroke; cardiovascular diseases

storage temp.

−196°C

General description

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Human Carotid Artery Smooth Muscle Cells (HCtASMC) provide a useful in vitro system to study all aspects of cardiovascular function and disease, especially those related to mechanisms of hyperplasia and hypertrophy of intimal smooth muscle cells leading to vascular occlusion in atherosclerosis and stent restenosis.Characterization: positive for smooth muscle cell specific alpha-actin expression.

Cell Line Origin

Artery

Application

cardiovascluar function, transfer of oxygenated blood to skull, brain, neck, face, effects of drugs

Components

Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

  • 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 10 doublings

Subculture Routine

Please refer to the HCtASMC Culture Protocol.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

3514-05A:

Certificates of Analysis (COA)

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Till Seime et al.
Cells, 10(6) (2021-06-03)
Calcification is a prominent feature of late-stage atherosclerosis, but the mechanisms driving this process are unclear. Using a biobank of carotid endarterectomies, we recently showed that Proteoglycan 4 (PRG4) is a key molecular signature of calcified plaques, expressed in smooth

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