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17751

Sigma-Aldrich

3-(1-Acetylacetonylazo)phthalhydrazide

BioReagent, suitable for fluorescence, ≥97.0% (HPLC)

Synonym(s):

3-(1,4-Dioxo-1,2,3,4-tetrahydrophthalazin-5-ylazo)-2,4-pentanedione

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About This Item

Empirical Formula (Hill Notation):
C13H12N4O4
Molecular Weight:
288.26
MDL number:
MFCD03791067
UNSPSC Code:
12352200
PubChem Substance ID:
57647610

description

luminescence test: Emmax475 nm 0.1M Tris pH 8.5; after addition of H2O2

product line

BioReagent

Assay

≥97.0% (HPLC)

mp

>300 °C

suitability

suitable for fluorescence

SMILES string

CC(=O)C(\N=N\c1cccc2C(=O)NNC(=O)c12)C(C)=O

InChI

1S/C13H12N4O4/c1-6(18)11(7(2)19)15-14-9-5-3-4-8-10(9)13(21)17-16-12(8)20/h3-5,11H,1-2H3,(H,16,20)(H,17,21)/b15-14+

InChI key

XWZORHXCJSIQCY-CCEZHUSRSA-N

Other Notes

Chemiluminescent diagnostic reagent for assaying Helicobacter pylori, which produces urease; much more sensitive than other methods

Pictograms

Skull and crossbones
GHS06

Signal Word

Danger

Hazard Statements

H301

Precautionary Statements

P301 + P310

Hazard Classifications

Acute Tox. 3 Oral

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

17751-50MG-F:
17751-VAR-F:
17751-BULK-F:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A Roda et al.
Analytical biochemistry, 264(1), 47-52 (1998-10-24)
A chemiluminescent urease activity assay has been developed and optimized using the chemiluminescent pH indicator phthalhydrazidylazoacetylacetone. This compound is stable at pH </= 7 and decomposes at higher pH values, emitting light in the presence of H2O2. Urease catalyzes hydrolysis

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