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14280

Sigma-Aldrich

BES buffered saline

BioUltra, for molecular biology, 2x concentrate

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

grade

for molecular biology

product line

BioUltra

form

liquid

quality

2x concentrate

composition

BES, 10.7 g/L
NaCl, 16.0 g/L
Na2HPO4, 0.27 g/L

impurities

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

refractive index

n20/D 1.338

pH

7.1 (25 °C)

cation traces

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.07
λ: 280 nm Amax: 0.02

application(s)

diagnostic assay manufacturing

Application

BES buffered saline can be used to study molecular biology, DNA and RNA purification, biological buffers and zwitterionic compounds. BES buffered saline has been used in a study that identified how to increase the transfection efficacy and subsequent long-term culture of resting human pancreatic duct epithelial cells.

Components

concentration (in H2O): 10.7 g/l BES, 16.0 g/l NaCl, 0.27 g/l Na2HPO4

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

14280-100ML:
14280-BULK:
14280-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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R Jesenofsky et al.
Pancreas, 17(3), 262-265 (1998-10-27)
Preparation of pancreatic duct epithelial cells from adult organs is possible by limited digestion and outgrowth of cells. These primary cells are mitotically active for only a short period. Therefore transfection with SV40 large-T antigen is one method to obtain
J. Sambrook et al.
Molecular Cloning: A Laboratory Manual, B10 (1989)
Karine Laulagnier et al.
Cellular and molecular life sciences : CMLS, 75(4), 757-773 (2017-09-29)
Amyloid beta peptide (Aβ), the main component of senile plaques of Alzheimer's disease brains, is produced by sequential cleavage of amyloid precursor protein (APP) and of its C-terminal fragments (CTFs). An unanswered question is how amyloidogenic peptides spread throughout the

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