11557

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

chromogenic, ≥98% (HPLC), powder or crystals

• Synonym(s): AzBTS-(NH₄)₂, Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
• Empirical Formula (Hill Notation): C₁₈H₂₄N₆O₆S₄
• CAS Number: 30931-67-0
• Molecular Weight: 548.68
• Beilstein: 7329461
• EC Number: 250-396-6
• MDL number: MFCD00010404
• UNSPSC Code: 12352204
• PubChem Substance ID: 329749485
• NACRES: NA.83

Properties

• Product Name: 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ≥98% (HPLC)
• Quality Level: 200
• Assay: ≥98% (HPLC)
• form: powder or crystals
• impurities: ≤6.0% water
• solubility: H₂O: 10 mg/mL, slightly hazy to clear
• storage temp.: 2-8°C
• SMILES string: [NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O
• InChI: 1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;
• InChI key: OHDRQQURAXLVGJ-AXMZSLBLSA-N

Description

Application

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt has been used:

• in the radical scavenging assay to test the antioxidant property of Pinus densiflora bark (PDB) extracts[1]
• to test the antioxidant capacity (AOC) of Aloe vera samples[2]
• as a substrate for streptavidin-horse radish peroxidase (HRPO) in enzyme-linked immunosorbent assay (ELISA)[3]

2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Other Notes

Chromogenic substrate for peroxidase[4]; The ABTS-H₂O₂-peroxidase system in EIA[5][6]

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves