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03285

Sigma-Aldrich

Cell Counting Kit

sufficient for 500 tests

Synonym(s):

Fluorescent Cell Counter

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About This Item

UNSPSC Code:
12352116
eCl@ss:
32161000
NACRES:
NA.32

form

liquid

usage

sufficient for 500 tests

storage temp.

−20°C

General description

Cell Counting kit, also known as FCCK, is a fluorescent cell counting product. The amount of a fluorescent dye, calcein, produced from Calcein-AM (3′,6′-Di(O-acetyl)-2′,7′-bis [N, Nbis-(carboxymethyl) aminomethyl]-fluorescein, tetraacetoxymethyl ester) by esterases in cells is directly proportional to the number of viable cells in a culture medium. Calcein-AM is highly lipophilic due to the presence of acetoxymethyl groups in the molecule, which rapidly permeates into the cell cytoplasm through the membrane.

Application

Cell Counting Kit or FCCK is used for the detection of live cells by fluorometric method.

Features and Benefits

Cell Counting Kit has the following advantages:

  • It doesn’t require any radioisotopes to function.
  • No solubilization procedure is needed.
  • Results are highly reproducible and accurate.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Please refer to KIT Component information

PRTR

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FSL

Please refer to KIT Component information

ISHL Indicated Name

Please refer to KIT Component information

ISHL Notified Names

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Cartagena Act

Please refer to KIT Component information

JAN Code

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Osteoporosis is a systemic metabolic bone disease during which bone mass decreases and bone quality is reduced. Maintaining the bone formation capacity of osteoblasts is crucial for the treatment of osteoporosis. In the present study, bioinformatics analysis was performed on
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In the pathogenesis of autoimmune disorders, the modulation of leukocytes' trafficking plays a central role, still poorly understood. Here, we focused on the effect of TLR2 ligands in trafficking of T helper cells through reshuffling of CD44 isoforms repertoire. Concurrently
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Laboratory investigation; a journal of technical methods and pathology, 101(2), 142-154 (2020-09-30)
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