W0625

Wright stain

≤4% purity (HPLC), powder

• Synonym(s): Eosin Methylene blue according to Wright
• CAS Number: 68988-92-1
• MDL number: MFCD00082143
• UNSPSC Code: 12171500
• NACRES: NA.47

Properties

• Product Name: Wright stain, suitable for blood stain
• Assay: ≤4% (HPLC)
• Quality Level: 200
• form: powder
• concentration: ≤4% (HPLC)
• technique(s): microbe id | staining: suitable
• color: dark green
• solubility: methanol: 10 mg/10 mL
• suitability: suitable for blood stain
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [s]5c6c(nc7c5cc(cc7)N(C)C)cc[c](c6)=[N+](C)C.Brc1c2[o+]c3c(c(c2cc(c1O)Br)c4c(cccc4)C(=O)O)cc(c(c3Br)O)Br
• InChI: 1S/C20H8Br4O5.C16H18N3S/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13/h1-6H,(H2-,25,26,27,28);5-10H,1-4H3/q;+1/p+1
• InChI key: AXIKDPDWFVPGOD-UHFFFAOYSA-O

Description

General description

Wright′s Stain solution is commercially available and is generally used in hematology. Its preparation involves heating methylene blue with sodium bicarbonate and combining the mixture with eosin. It is alkaline, hence, its concentration is continuously changing due to the progressive oxidizing action of the alkali. Therefore, Giemsa′s stain is added to preserve the azurophilic properties of polychrome dyes.

Application

Wright′s Stain is mainly used for the staining of peripheral blood smears and bone marrow smears.

• It is also used for G-banding and helps in the analysis of poorly spread metaphases.
• It has been used to study the cytogenetics of human hematopoietic cells.[1]
• It has also been employed in the karyotyping of human embryonic stem cells.[2]
• It is also used in the staining of chromosomes.
• It is used for the exudate analysis of white blood cell concentration.

Reported to be superior to May-Grunwald Stain in blood staining.

Features and Benefits

The purified dyes in Wright′s Stain eliminate inconsistent staining and yield reproducible lot-to-lot chromogenic responses.

Preparation Note

Prepared using polychromed (oxidized) methylene blue and eosin Y.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H302,H319
• Precautionary Statements: P280 - P301 + P312 + P330 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves