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S5133

Sigma-Aldrich

Schiff′s fuchsin-sulfite reagent

liquid

Synonym(s):

Schiff reagent

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500 ML
¥12,300
1 L
¥21,200

¥12,300

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500 ML
¥12,300
1 L
¥21,200

About This Item

Empirical Formula (Hill Notation):
C20H21N3O3S
Molecular Weight:
383.46
UNSPSC Code:
12171500
NACRES:
NA.47

¥12,300

In StockDetails

Request a Bulk Order

Product Name

Schiff′s fuchsin-sulfite reagent, suitable for detection of glycoproteins

Assay

3-5 mM iodine (I) basis (titration)

Quality Level

100

form

liquid

technique(s)

microbe id | staining: suitable

color

colorless

solubility

water: soluble

density

0.990 g/cm3

suitability

suitable for detection of glycoproteins

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

General description

Schiff’s fuchsin-sulfite reagent is a part of the glycoprotein detection kit. It is primarily used in the Periodic Acid-Schiff (PAS) staining method to detect and visualize aldehydes, glycoproteins in biological samples. The reagent consists of basic fuchsin dye and sodium bisulfite. It reacts with the aldehyde groups of carbohydrates and produces a pink to magenta color, allowing for the identification of these substances in samples.

Application

Schiff′s fuchsin-sulfite reagent has been used:

  • in periodic acid-Schiff (PAS) staining to determine goblet cells and salivary glycopreoteins like mucins
  • to stain ovaries and testis[1]
  • to stain tomato and Medicago primary roots[2]
  • to stain undifferentiated sarcomas (USARCs)[3]
It provides a system to easily detect the sugar moieties of glycoproteins on SDS PAGE or Western Blotting membranes.

Preparation Note

Prepared using pararosaniline.

Pictograms

Health hazardCorrosion
GHS08,GHS05

Signal Word

Danger

Hazard Statements

H290,H350

Hazard Classifications

Carc. 1B - Met. Corr. 1

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

S5133-1L-KC:
S5133-VAR:
S5133-1L:4548173951331
S5133-BULK:
S5133-500ML:4548173951348

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Certificates of Analysis (COA)

Lot/Batch Number
SHBR2337
SHBP9152
SHBN6996
MKCN1525
MKCM1419

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Functionality of Soy Protein Produced
by Enzyme-Assisted Extraction
S Jung
J. Oil Colour Chem. Assoc. (2006)
Crisalejandra Rivera-Perez et al.
Scientific reports, 10(1), 20201-20201 (2020-11-21)
Nacre is the main component of the pearl oyster shells and it is synthesized by specialized soluble and insoluble shell matrix proteins. Insoluble proteins from the decalcification of the shell are the less studied proteins due to the technical problems
Undifferentiated Sarcomas Develop through Distinct Evolutionary Pathways
Steele CD, et al.
Cancer Cell, 35(3), 441-456 (2019)
Identification of salivary proteins at oil-water interfaces stabilized by lysozyme and ?-lactoglobulin
Erika Silleti
Journal of Archaeology of Egypt/Egyptology (2010)
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