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M0553

Millipore

Middlebrook ADC Growth Supplement

Enrichment supplement recommended for the cultivation of Mycobacteria

Synonym(s):

Middlebrook Albumin Dextrose Catalase Supplement

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About This Item

UNSPSC Code:
41171614
NACRES:
NA.85

Quality Level

200

sterility

sterile (Filtered and Aseptic Handled)

form

liquid

shelf life

limited shelf life, expiry date on the label

application(s)

clinical testing
environmental
food and beverages
life science and biopharma
microbiology
veterinary

storage temp.

2-8°C

suitability

mycobacteria

General description

Middlebrook ADC growth supplement is used to isolate a wide variety of Mycobacteria including M. tuberculosis. It contains Bovine albumin, dextrose, and catalase. Bovine albumin neutralizes the toxic products. Dextrose is the fermentable sugar and catalase cleaves the toxic hydrogen peroxides which creates stress to the Mycobacteria cells thus, making it an ideal media for the cultivation of Mycobacterial species

Application

Specialized culture media supplement, designed to isolate and cultivate various Mycobacterial species.

Components

Ingredients: (per vial)
Bovine albumin fraction V 2.5g
Dextrose 1.0g
Catalase 0.0015g
Distilled water 50 ml

Reconstitution

Warm up the refrigerated supplement to 45-50°C. Aseptically add the contents of the vial to 450 ml of sterile Middlebrook 7H9 Broth Base (M0178). Mix well and pour into sterile screw capped tubes.

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

M0553-1VL-BULK:
M0553-1VL:
M0553-BULK:
M0553-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM1974
BCCK4936
BCCJ9945
BCCJ5055
BCCJ3872

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Nancy Patience Motlalepula Komape et al.
BMC complementary and alternative medicine, 17(1), 128-128 (2017-02-27)
Tuberculosis is an infectious communicable disease and the causative agent of the disease has over the years developed resistance to streamline chemotherapeutic agents with dire consequences and there is a need for development of new and more potent alternatives. Constituents
Joshua N Gustine et al.
Current microbiology, 76(4), 398-409 (2019-01-04)
Cell wall hydrolases are enzymes that cleave bacterial cell walls by hydrolyzing specific bonds within peptidoglycan and other portions of the envelope. Two major sources of hydrolases in nature are from hosts and microbes. This study specifically investigated whether cell

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