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F5415

Sigma-Aldrich

Ficoll® solution

Type 400, 20% in H2O

Synonym(s):

Ready-to-use density gradient media

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75

sterility

0.2 μm filtered

Quality Level

type

Type 400

form

liquid

concentration

20% in H2O

color

clear colorless

solubility

water: soluble

density

1.055-1.075 g/mL

application(s)

hematology
histology

storage temp.

2-8°C

General description

Ficoll® Solution is a highly branched polymer of sucrose, also known as polydisperse mixtures of dextran. Ficoll® molecules are inert and not re-absorbed by proximal tubules. The structure is open and deformable, and not a rigid sphere thereby becoming a preferred choice in “crowding” experiments.
Used in electrophoresis and as a hapten carrier. Most commonly used to prepare density gradients.

Application

Ficoll® has been used in


  • Isolation of cord blood mononuclear cells
  • Isolation of CD133 negative cells
  • Isolation and expansion of circulating endothelial progenitor cells.

Other Notes

A nonionic synthetic polymer of sucrose.

Legal Information

Ficoll is a registered trademark of Cytiva

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

F5415-50ML:
F5415-25ML-PW:
F5415-25ML:
F5415-PH:
F5415-VAR:
F5415-50ML-PW:
F5415-BULK:
F5415-VAR-PW:


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Protocols

Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.

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