93745

TSC Agar

suitable for microbiology, NutriSelect^® Basic

• Synonym(s): Tryptose Sulfite Cycloserine Agar
• UNSPSC Code: 41171606
• NACRES: NA.74

Properties

• Agency: according to ISO 15213:2003; according to ISO 7937:2004
• Quality Level: 200
• sterility: non-sterile
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15 g/L ; ammonium ferric citrate, 1 g/L ; meat peptone (peptic), 15 g/L ; sodium disulfite, 1 g/L ; soya peptone, 5 g/L ; yeast extract, 5 g/L
• manufacturer/tradename: NutriSelect^® Basic
• technique(s): microbiological culture: suitable
• final pH: 7.6±0.2 (25 °C)
• application(s): agriculture; clinical testing; environmental; food and beverages; pathogen testing; water monitoring; microbiology
• suitability: selective and differential for Clostridium spp.

Description

Application

For the isolation and enumeration of vegetative forms as well as spores from Clostridium perfringens in food, clinical specimens and other material acc. to Harmon et al. (1971).

Preparation Note

Base Agar:
Dissolve 42 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cooling to approx. 50°C.

TSC Agar:
To 1 litre Base Agar add 400 mg rehydrated D-cycloserine (Cat. No. 30020) as a sterile filtered aqueous solution or 2 vials of Perfringens T.S.C. Supplement (Cat. No. P9352) and 50 ml Egg Yolk Emulsion (Cat. No. 17148). Mix well and pour into petri plates.

TSC Agar egg yolk free (also used as overlay agar):
Add 400 mg rehydrated D-cycloserine (Cat. No. 30020) or 2 vials of Perfringens T.S.C. Supplement (Cat. No. P9352) to 1 litre Base Agar and mix well.

TSC Fluorescence Agar:
Add to 1 litre Base Agar 5.5 g rehydrated TSC Agar supplement (Cat. No. 80548) as a sterile filtered aqueous solution. Mix well and pour plates.

SFP Agar (Shahidi-Ferguson Perfringens Agar):
To 1 litre Base Agar 2 vials of Perfringens S.F.P. Supplement Cat. No. P9477) and 50 ml Egg Yolk Emulsion (Cat. No. 17148) are added. Mix well and pour into sterile petri plates.

Other Notes

Isolation and enumeration of sulphite-reducing Clostridium spores and Group D faecal streptococci from oysters [1][2]; Membrane filtration enumeration of faecal clostridia and C. perfringens in water[3]

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319
• Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves