39734

Membrane Lactose Glucuronide Agar

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): MLGA, m-Lauryl Sulfate Chromogen Agar
• UNSPSC Code: 41171606
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 10 g/L ; X-glucuronide (BCIG), 0.2 g/L ; lactose, 30 g/L ; peptone, 40 g/L ; phenol red, 0.2 g/L ; sodium lauryl sulfate, 1.0 g/L ; sodium pyruvate, 0.5 g/L ; yeast extract, 6 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 7.4±0.2 (25 °C)
• application(s): agriculture; bioburden testing; clinical testing; environmental; food and beverages; water monitoring; microbiology
• suitability: selective and differential for Enterobacter spp.; selective and differential for Escherichia coli; selective and differential for Klebsiella spp.; selective and differential for coliforms; selective and differential for enterovirulent E. coli (EEC)

Description

Application

A chromogenic medium for the differentiation and enumeration of Escherichia coli and other coliforms, which simplifies the membrane filtration technique by reducing the number of filtration stages and the need for further confirmation steps. This medium is described in the Environment Agency′s report of UK, ′Methods for Examination of Waters and Associated Material - The Microbiology of Drinking Water (2002) - Part 4 - Methods for the isolation and enumeration of coliform bacteria and Escherichia coli (including E. coli O157:H7).

Preparation Note

Suspend 88g in 1 litre of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C and pour into sterile Petri dishes.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves