Skip to Content
Merck
All Photos(1)

Documents

Safety Information

30911

Sigma-Aldrich

Denaturation solution for the isolation of RNA

BioUltra, for molecular biology

Synonym(s):

RNA isolation, denaturation

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD00217302
UNSPSC Code:
12352202
NACRES:
NA.25

grade

for molecular biology

Quality Level

100

product line

BioUltra

composition

guanidine thiocyanate, 4 M
Na-citrate, 25 mM
sarcosyl, 0.5%

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

refractive index

n20/D 1.433

pH

5.5-7.0

anion traces

chloride (Cl-): ≤500 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤2 mg/kg
As: ≤0.1 mg/kg
Ba: ≤2 mg/kg
Bi: ≤2 mg/kg
Ca: ≤10 mg/kg
Cd: ≤2 mg/kg
Co: ≤2 mg/kg
Cr: ≤2 mg/kg
Cu: ≤2 mg/kg
Fe: ≤2 mg/kg
K: ≤100 mg/kg
Li: ≤2 mg/kg
Mg: ≤2 mg/kg
Mn: ≤2 mg/kg
Mo: ≤2 mg/kg
Ni: ≤2 mg/kg
Pb: ≤2 mg/kg
Sr: ≤2 mg/kg
Zn: ≤2 mg/kg

λ

neat

UV absorption

λ: 280 nm Amax: 1

Application

Denaturation solution for the isolation of RNA is a guanidine thiocyanate, sarcosyl based protein denaturization solution useful for the purification of mRNA.

Other Notes

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Statements

H302 + H332,H314,H412

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

Supplementary Hazards

EUH032

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

30911-100ML:
30911-VAR:
30911-BULK:

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCF1640
BCBS3081V
BCBK2156V
BCBH0592V
BCBG7029V

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Y L Tsai et al.
Applied and environmental microbiology, 57(3), 765-768 (1991-03-01)
A protocol for direct extraction of mRNA from soil samples was developed. Soil samples (10 g) were washed twice with 120 mM phosphate buffer (pH 5.2). The lysis of cells, fixation of RNA, and hydrolysis of DNA were achieved by
P Chomczynski et al.
Analytical biochemistry, 162(1), 156-159 (1987-04-01)
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service