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216313

Sigma-Aldrich

Methylene Violet (Bernthsen)

certified by the Biological Stain Commission, Dye content ≥65 %

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About This Item

Empirical Formula (Hill Notation):
C14H12N2OS
CAS Number:
2516-05-4
Molecular Weight:
256.32
Colour Index Number:
52041
EC Number:
219-733-4
MDL number:
MFCD00005064
UNSPSC Code:
12171500
PubChem Substance ID:
24852949
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

100

form

powder

composition

Dye content, ≥65%

mp

216 °C (dec.) (lit.)

solubility

soluble, clear

λmax

580 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CN(C)c1ccc2N=C3C=CC(=O)C=C3Sc2c1

InChI

1S/C14H12N2OS/c1-16(2)9-3-5-11-13(7-9)18-14-8-10(17)4-6-12(14)15-11/h3-8H,1-2H3

InChI key

ALJHHTHBYJROOG-UHFFFAOYSA-N

Suitability

Certified for use as a constituent of MacNeal′s tetrachrome stain for blood.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

216313-10G:
216313-VAR:
216313-BULK:
216313-1G:

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Certificates of Analysis (COA)

Lot/Batch Number
17196PH
04910HT
21202HB
07914AY
04421LS

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Jürgen Burhenne et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 863(2), 273-282 (2008-02-09)
Selective and sensitive methods for the determination of the cationic dye and anti-malarial methylene blue in human liquid whole blood, dried whole blood (paper spot), and plasma depending on protein precipitation and cation exchange chromatography coupled to electrospray ionisation (ESI)
Kevin E Washburn et al.
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 68(2), 105-111 (2004-06-11)
The objectives of this study were to determine the optimal concentration of phenothiazine dye required to inactivate bovine viral diarrhea virus (BVDV) in goat colostrum following 60 min of illumination and determine if immunoglobulin concentration is affected by this technique.
C S Sastry et al.
The Analyst, 114(4), 513-515 (1989-04-01)
A fairly sensitive spectrophotometric method for the determination of ibuprofen, ketoprofen, piroxicam, diclofenac sodium, mefenamic acid or enfenamic acid in bulk samples and pharmaceutical preparations is described, based on the formation of a chloroform-soluble, coloured ion-association complex between the drug
H Morrison et al.
Photochemistry and photobiology, 66(2), 245-252 (1997-08-01)
The interaction of methylene violet (MV) and 4-bromomethylene violet (BrMV) with calf thymus and supercoiled phi X174 phage RF I DNA is reported. Measurements employing UV-visible absorption spectroscopy and equilibrium dialysis give evidence for the formation of complexes by each
A Skripchenko et al.
Photochemistry and photobiology, 65(3), 451-455 (1997-03-01)
Previous studies with methylene blue (MB) in red cell suspensions have demonstrated that extracellular, but not intracellular, virus can be readily photoinactivated. To test if the resistance of intracellular virus to inactivation is related to the permanent positive charge of
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