KK3009
Roche
KAPA Taq EXtra PCR Kit
Synonym(s):
PCR, Taq
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About This Item
UNSPSC Code:
41106300
NACRES:
NA.55
Recommended Products
usage
sufficient for 500 reactions
Quality Level
feature
High Fidelity PCR
Long Range PCR
dNTPs included
hotstart: no
packaging
pkg of 250 U
manufacturer/tradename
Roche
technique(s)
PCR: suitable
input
purified DNA
storage temp.
−20°C
Application
The KAPA Taq EXtra PCR system is ideally suited for:
- PCR of short- and medium-length targets (<5 kb)
- Long-range PCR (5–15 kb)
- PCR with limiting amounts of template DNA.
Biochem/physiol Actions
The KAPA Taq EXtra PCR system is a blend of KAPA Taq DNA polymerase and a modified archaeal (Type B) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support robust, long range, and sensitive PCR. Both enzymes possess 5′→3′ polymerase activity, but only KAPA Taq DNA polymerase possesses 5′→3′ exonuclease activity and only the Type B DNA polymerase possesses 3′→5′ exonuclease (proofreading) activity. Reaction products generated with KAPA Taq EXtra are polyA-tailed, and can be cloned into TA-cloning vectors.
Features and Benefits
- KAPA Taq EXtra is a blend of KAPA Taq and a proofreading DNA polymerase.
- Suitable for short-, medium- and long-range PCR, and PCR with low amounts of template DNA.
- Fidelity is 2-4 times better than Taq
- For short amplicons, replace Taq DNA polymerase for improvements in yield and/or fidelity.
- Amplify mid- to long-range targets with high yield and sensitivity.
- KAPA Taq EXtra Buffer (Mg-free) is designed for long-range, sensitive PCR. MgCl2 is supplied separately for optimization.
- The fidelity of KAPA Taq EXtra is 2-4 fold higher than that of KAPA Taq DNA Polymerase, but is lower than the fidelity of pure proofreading polymerases, such as KAPA HiFi.
Quality
Each batch of KAPA Taq EXtra DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA Taq EXtra PCR kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage.
Other Notes
For Research Use Only. Not for use in diagnostic procedures.
Kit Components Only
Product No.
Description
- KAPA Taq EXtra DNA Polymerase 5 U/µL
- KAPA Taq EXtra Buffer (5X)
- KAPA dNTP Mix 10 mM each
- MgCl2 25 mM
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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