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Key Documents

COA/COQ

Safety Information

Safety & Regulatory

UC0E05

UCOE^® Intermediate Vector Set

Name: UCOE<SUP>®</SUP> Intermediate Vector Set
Brand: MM

Synonym(s):

UCOE Mammalian Protein Expression Vectors, UCOE Mammalian Protein Expression Plasmids, Ubiquitous Chromatin Opening Element, UCOE05

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About This Item

UNSPSC Code:

41106621

eCl@ss:

34360190

NACRES:

NA.51

Pricing and availability is not currently available.

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Sigma-Aldrich

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P4832

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P3B030D10

Pellicon^® 3 Cassette with Biomax^® Membrane

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P1274

Poly-L-lysine hydrobromide

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SML0770

P5091

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P5899

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S45900

QUINOLINE-2-CARBOXYLIC ACID (2-CHLORO-ETHYL)-AMIDE

shipped in

dry ice

Quality Level

100

General description

The Ubiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression. Unlike traditional vectors, UCOE-containing vectors have the capacity to ease the process of isolating cell line clones that express high-levels of recombinant proteins. UCOE sequences promote reproducible and stable high level expression of a linked gene by altering chromatin structure to a transcriptionally permissive state that negates epigenetic-mediated (e.g. DNA methylation) silencing.

CET 1019 contains the 3 kb mouse Rps3 UCOE and the 1.5 kb fragment of human HNRPA2B1-CBX3 UCOE.
CET 1310 contains the 8 kb fragment of human HNRPA2B1-CBX3 UCOE.
CET 1316 contains the 4 kb fragment of human HNRPA2B1-CBX3 UCOE.

References
Antoniou, M., et al.. (2003) Transgenes Encompassing Dual Promoter CpG Islands from the Human TBP and HNRPA2B1 Loci are Resistant to Heterochromatin-mediated Silencing. Genomics, 82: 269-279.
Antoniou, M., et al. (2010) A Ubiquitous Chromatin Opening Element (UCOE) Confers Resistance to DNA Methylation-mediated Silencing of Lentiviral Vectors. Mol Ther, 18: 1640-1649.

THIS PRODUCT IS ONLY AVAILABLE FOR SALE TO ACADEMIC INSTITUTIONS OR NOT-FOR-PROFIT ENTITIES FOR USE UNDER THIS PRODUCT LABEL LICENSE. FOR INFORMATION ON COMMERCIAL LICENSING OF THE PATENTED UCOE TECHNOLOGY, PLEASE CONTACT [email protected].

Components

1) 10ug CET 1019 (3 kb Rps3 and 1.5kb HNRPA2B1-CBX3) Vector (CS221300)

2) 10ug CET 1310 (8 kb HNRPA2B1-CBX3) Vector (CS221301)

3) 10ug CET 1316 (4 kb HNRPA2B1-CBX3) Vector (CS221302)

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

UCOE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.