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Safety Information

ST1711

Sigma-Aldrich

Anti-DFHR Mouse mAb (2B10)

liquid, clone 2B10, Calbiochem®

Synonym(s):

Anti-Dihydrofolate Reductase

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

2B10, monoclonal

form

liquid

does not contain

preservative

species reactivity

rat, human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG2a

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DHFR(1719)

General description

Purified mouse monoclonal antibody. Recognizes the ~20-22 kDa DHFR protein.
Recognizes the ~20-22 kDa DHFR protein in HeLa and PC-12 cells.
This Anti-DFHR Mouse mAb (2B10) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of DFHR.

Immunogen

A recombinant polypeptide corresponding to amino acids of 88-188 human DHFR, expressed as a GST fusion protein

Application




ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
293T cells
Positive Control
HeLa cells, PC-12 Cells

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Vodenicharov, M.D., et al. 2010. The EMBO Journal29, 3007.
Ionova, I.A., et al. 2008. Am. J. Physiol. Heart Circ. Physiol.295 H2178.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

ST1711-100UG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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