SM-2004

Low Trypsin-High EDTA

PBS Based, 0.025% Trypsin & 0.75mM EDTA (1X), without Ca 2+ & Mg 2+

• Synonym(s): Low Trypsin-High EDTA Solution, PBS-Based Trypsin Solution, Trypsin-EDTA PBS
• UNSPSC Code: 12352204
• eCl@ss: 32160801
• NACRES: NA.75

Properties

• Product Name: Low Trypsin-High EDTA, PBS Based, 0.025% Trypsin and 0.75mM EDTA (1X), without Ca²⁺ and Mg²⁺, The Low Trypsin-High EDTA, PBS Based, 0.025% Trypsin & 0.75mM EDTA (1X), without Ca 2+ & Mg 2+ is available in a 100 mL format.
• Quality Level: 200
• form: liquid
• manufacturer/tradename: Specialty Media
• input: sample type: human embryonic stem cell(s); sample type induced pluripotent stem cell(s); sample type epithelial cells; sample type hematopoietic stem cell(s); sample type mesenchymal stem cell(s); sample type neural stem cell(s); sample type pancreatic stem cell(s); sample type: mouse embryonic stem cell(s); sample type cardiac stem cell(s)

Description

Application

Low Trypsin-High EDTA, PBS Based, 0.025% Trypsin and 0.75mM EDTA (1X), without Ca²⁺ and Mg²⁺ has been used:

• as an incubating medium for single-cell preparation of human islets[1]
• in the preparation of mouse embryonic fibroblast cells stock for conventional gene targeting[2]
• to trypsinize human umbilical vein endothelial cells (HUVECs) for coating the beads with HUVECs[3]

Storage and Stability

Store at -20°C for 18 months.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable