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SCT120

Sigma-Aldrich

BioTracker 488 Green Nuclear Dye

Live cell imaging green nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.

Synonym(s):

Live cell imaging probe, Live cell nuclear imaging probe

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.47

technique(s)

cell based assay: suitable

detection method

fluorometric

shipped in

ambient

General description

The nucleus is a membrane-enclosed organelle found in eukaryotic cells. Cell nuclei contain most of the cell′s genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. Traditionally, DNA stains such as DAPI and Hoechst have been used for microscopy but require fixation and cannot be used for live cell imaging.

The BioTracker 488 Green Nuclear Dye is a cell-membrane permeable green fluorescent DNA dye that specifically stains nuclei in live or fixed cells. It has excellent specificity for DNA without the need for a wash step, and it has low toxicity for live cell imaging. The dye is supplied with a vial of verapamil, an efflux pump inhibitor that may improve probe retention and live cell staining in certain cell types.

Note: BioTracker Nuclear Dyes also shows blue fluorescence in the DAPI channel, and may not be suitable for multicolor imaging with blue probes.

Spectral Properties
Absorbance: 500nm
Emission: 515nm

Application

Live cell fluorescent imaging
Live cell imaging green nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.
Research Category
Cell Imaging
Research Sub Category
Live Cell Dye

Components

1) 1 vial of 50µL BioTracker 488 Green Nuclear Dye (1000X in DMSO) (CS224594)

2) 1 vial of 100µL Verapamil HCL (100mM in DMSO) (CS224592)

Quality

Spectral Properties
Absorbance: 500nm
Emission: 515nm

Physical form

Liquid

Storage and Stability

Store BioTracker 488 Green Nuclear Dye at 2-8ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H302,H411

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2

Storage Class Code

10 - Combustible liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SCT120:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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