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Safety Information

SAE178-100UG

Sigma-Aldrich

SAvPhire Monomeric Streptavidin iFluor 647

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

clone

monoclonal

Quality Level

200

species reactivity

all

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

General description

Upon reconstitution in 100 mL of distilled water the concentration will be 1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

SAvPhire Monomeric Streptavidin expressed in E. coli and conjugated with iFluor 647 that is suitable for immunodetection of biotinylated proteins.

Application

Epitope: Unknown

Quality

Evaluated by Immunocytochemistry in A431 cells.

Immunofluorescent analysis of A431 cells performed using a 1:1 mixture of biotinylated Anti-EGFR (1:1,000 dilution) and SAvPhire Monomeric Streptavidin iFluor 647 detected EGFR.

Physical properties

15.5 kDa for SAvPhire Monomeric Streptavidin (with an N-terminal His-tag and a C-terminal FLAG tag).

Physical form

SAvPhire Monomeric Streptavidin Alpha Fluor 647 lyophilized from PBS containing D-Mannitol and Sucrose without azide.

Storage and Stability

Stable for 1 year at +2°C to +8°C from date of receipt.

Other Notes

Predicted to react with all species.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SAE178-100UG:

Certificates of Analysis (COA)

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