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MABS1927

Sigma-Aldrich

Anti-CD173 and CD174 Antibody, clone BRIC 231

clone BRIC 231, from mouse

Synonym(s):

Fucosylated histo-blood group antigens H type 2/Lewis Y, Clone BRIC 231

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

BRIC 231, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... FUT4(2526)

General description

CD173 (also known as fucosylated histo-blood group antigens H type 2; Blood group O) and CD174 (also known as Lewis Y or LeY) are developmentally regulated cell surface carbohydrate antigens that are expressed to varying degrees on many human carcinomas. They are also expressed on CD34+ hematopoietic progenitor cells, but are absent on mature lymphocytes. Hence, it is suggested that CDS173 and CD174 play a role in homing process of hematopoietic stem cells. Fucosylated glycans are known to be involved in cell-cell and cell-matrix interactions, differentiation, cell proliferation, and apoptosis. CD173 is a monofucosylated H2 where as CD174 is a difucosylated LeY. They are structurally related and have 2 precursor chains (Galbeta1-4GlcNAc-) in common. CD173 is synthesized through the addition of a fucose on type 2 precursor chains by the alpha 1-2 fucosyltransferases. CD174 results from the epistatic interaction on type 2 precursor chains, of the products of the alpha 1-2 fucosyltransferases and alpha 1-3 fucosyltransferases.

Specificity

Clone BRIC231 detects CD173/CD174 in human cells.

Immunogen

Whole HEL cells

Application

Anti-CD173 CD174, clone BRIC 231, Cat. No. MABS1927, is a mouse monoclonal antibody that detects CD173/CD174 in human and is tested for use in Flow cytometry and Immunocytochemistry.
Immunocytochemistry Analysis: A representative lot detected CD173 CD174 in Immunocytochemistry applications (Cao, Y., et. al. (2001). Glycobiology. 11(8):677-83).

Flow Cytometry Analysis: A representative lot detected CD173 CD174 in Flow Cytometry applications (Cao, Y., et. al. (2001). Glycobiology. 11(8):677-83).
Research Category
Signaling

Quality

Evaluated by Immunocytochemistry in KG1 cells.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected CD173 CD174 in KG1 cells.

Target description

59.08 kDa calculated.

Physical form

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MABS1927:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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