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Safety Information

MABS1720

Sigma-Aldrich

Anti-Acetyl-CoA Synthetase Antibody, clone 6C1.2

clone 6C1.2, from mouse

Synonym(s):

Acetyl-coenzyme A synthetase, cytoplasmic, Acetate-CoA ligase, Acetyl-CoA synthetase, AceCS, ACS, Acyl-activating enzyme, Acyl-CoA synthetase short-chain family member 2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6C1.2, monoclonal

species reactivity

human

technique(s)

western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_061147

UniProt accession no.

Q9NR19

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... ACSS2(55902)

General description

Acetyl-coenzyme A synthetase, cytoplasmic (EC 6.2.1.1; UniProt Q9NR19; also known as Acetate-CoA ligase, Acetyl-CoA synthetase, AceCS, ACS, Acyl-activating enzyme, Acyl-CoA synthetase short-chain family member 2) is encoded by the ACSS2 (also known as ACAS2) gene (Gene ID 55902) in human. ACS plays a vital role in prokaryotic and eukaryotic cells by activating acetate to form acetyl CoA that serves as a source of two carbon metabolite for lipid synthesis and energy producing reactions. It is also reported to play a role in gene expression. In yeast cells it delivers acetyl CoA for histone acetylation. ACS contributes to cancer cell growth under low-oxygen and lipid-depleted conditions and its expression is upregulated under metabolically stress. Silencing ACSS2 gene is shown to reduce the growth of tumor xenografts. Ref.: Starai, VJ., and Escalante-Semerena, JC. (2004). Cell. Mol. Life Sci. 61, 2020 2030. Schug, ZT et al. (2015). Cancer Cell. 27:57-71.

Specificity

Immunogen sequence is present in both spliced isoforms of human acetyl-CoA synthetase reported by UniProt (Q9NR19).

Immunogen

GST-tagged recombinant human acetyl-CoA synthetase N-terminal fragment.

Application

Detect Acetyl-CoA synthetase using this mouse monoclonal Anti-Acetyl-CoA Synthetase Antibody, clone 6C1.2, Cat. No. MABS1720, validated for use in Western Blotting.

Quality

Evaluated by Western Blotting in HEPG2 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected acetyl-CoA synthetase in 10 µg of HepG2 cell lysate.

Target description

~80 kDa observed. 78.58/80.09 kDa (isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MABS1720:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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