MABN770

Anti-Melanopsin Antibody, clone 1F8.1

clone 1F8.1, from mouse

• Synonym(s): Melanopsin, Opsin-4
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1F8.1, monoclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG2aλ
• NCBI accession no.: NP_150598
• UniProt accession no.: Q9UHM6
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... OPN4(94233)

Description

General description

Melanopsin (UniProt Q9UHM6; also known as Opsin-4) is encoded by the OPN4 (also known as MOP) gene (Gene ID 94233) in human. Opsins belong to the superfamily of G protein-coupled receptors (GPCRs). Melanopsin is a blue light-sensitive opsin protein that is capable of rendering cells intrinsically light responsive. Melanopsin expression defines a population of retinal ganglion cells that are intrinsically photosensitive (ipRGCs). Melanopsin allows mammals to measure irradiance for centrally mediated responses such as circadian entrainment. In addition, the information generated by melanopsin is used by the visual system as a signal for light adaptation. Using melanopsin-dependent phototransduction mechanism coupled with extrinsic signals originating with rods and cones, ipRGCs can encode light levels over many decimal orders, allowing the visual system to adapt to a broad range of light intensity from starlight to broad daylight.

Specificity

Clone 1F8.1 is expected to react with both spliced isoforms of human melanopsin.

Immunogen

GST-tagged recombinant protein corresponding to human Melanopsin C-terminal sequence

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Melanopsin in human retina tissue.

This Anti-Melanopsin Antibody, clone 1F8.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Melanopsin .

Quality

Evaluated by Western Blotting in human retinal pigment epithelia (RPE) ARPE-19 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Melanopsin in 10 µg of human retinal pigment epithelia (RPE) ARPE-19 cell lysate.

Target description

~52 kDa observed

Physical form

Format: Purified

Purified mouse monoclonal IgG2aλ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable