MABN2708

Anti-Islet-1/2 homeobox Antibody, clone 39.4D5

• Synonym(s): ISL LIM homeobox 1, ISL LIM homeobox 2, Insulin gene enhancer protein ISL-1, Insulin gene enhancer protein ISL-2, Islet-1, Islet-2
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 39.4D5, monoclonal
• mol wt: calculated mol wt 39.04 kDa; observed mol wt ~43 kDa
• purified by: using protein G
• species reactivity: human, rat, chicken, mouse, zebrafish, frog
• packaging: antibody small pack of 100
• technique(s): ChIP: suitable; electron microscopy: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG2bκ
• epitope sequence: C-terminal
• Protein ID accession no.: NP_059035
• UniProt accession no.: P61374
• storage temp.: 2-8°C
• Gene Information: rat ... , Isl2(64444)

Description

Specificity

Clone 39.4D5 is a mouse monoclonal antibody that specifically detects Islet-1/2 homeobox. It targets an epitope within 172 amino acids from the C-terminal region.

Immunogen

C-terminal recombinant protein of rat Islet-1.

Application

Quality Control Testing

Evaluated by Western Blotting in rat hypothalamus tissue lysate.

Western Blotting Analysis: A 1:1000 dilution of this antibody detected Islet-1/2 homeobox in rat hypothalamus tissue lysate.

Tested Applications

Flow Cytometry Analysis: A representative lot detected Islet-1 and 2 homeobox in Flow Cytometry applications (Lei, I., et al. (2020). Front Cell Dev Biol. 7: 358).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Islet-1 and 2 homeobox in Chromatin Immunoprecipitation applications (Du, A., et al. (2009). Diabetes. 58(9): 2059-69; Ma, Q., et al. (2020). Cell Rep. 31(3): 107532).

Immunocytochemistry Analysis: A representative lot detected Islet-1 and 2 homeobox in Immunocytochemistry applications (Wang, Y., et al. (2019). Nat Commun. 10(1): 4137; Carvelli, A., et al. (2022). EMBO J. 41(13): e108918).

Western Blotting Analysis: A representative lot detected Islet-1 and 2 homeobox in Western Blotting applications (Baleriola, J., et al. (2016). Sci Rep. 6: 25928).

Electrophoretic Mobility Shift Assay: A representative lot detected Islet-1 and 2 homeobox in Electrophoretic Mobility Shift Assay applications (Du, A., et al. (2009). Diabetes. 58(9);:2059-69).

Immunohistochemistry Applications: A representative lot detected Islet-1/2 homeobox in Immunohistochemistry applications (Chen, Y.Y., et al. (2007). J Neurosci. 27(31): 8448-56; Du, A., et al. (2009). Diabetes. 58(9): 2059-69; Bielle, F., et al. (2011). Neuron. 69(6):1085-98; Schick, E., et al. (2019). Sci Rep. 9(1):9358; Bornhorst, D., et al. (2019). Nat Commun. 10(1): 4113; Vieira, J.R., et al. (2022). Neuron. 110(24): 4074-4089).

Immunofluorescence Analysis: A representative lot detected Islet-1/2 homeobox in Immunofluorescence applications (Du, A., et al. (2009). Diabetes. 58(9): 2059-69; Wang, Y., et al. (2019). Nat Commun. 10(1):4137; Vieira, J.R., et al. (2022). Neuron. 110(24): 4074-4089).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Insulin gene enhancer protein ISL-1 (UniProt: P61374; also known as Islet-1) is encoded by the Isl1 gene (Gene ID: 64444) in rat. Islet-1 is a DNA-binding transcriptional activator that is predominantly localized in nucleus and, expressed in pancreatic islets and is essential for the maturation, proliferation, and survival of the endocrine pancreas. It also plays a crucial role in the development of various tissues, including the heart, motor neurons, and sensory neurons. It recognizes and binds to the consensus octamer binding site 5′-ATAATTAA-3′ in promoter of target genes. Its DNA binding region is localized in amino acids 181-240. Islet-1 also contains two LIM Zinc-binding domains (aa 17-70 and 79-133) and a LIM-binding domain (aa 262-291). Two isoforms of islet-1 have been described that are produced by alternative splicing. It plays a key role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. It cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. It is also involved in the specification of motor neurons in cooperation with LHX3 and LDB1. It is reported to be essential for cardiac development and is considered as a marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells. It also controls the expression of fibroblast growth factor and bone morphogenetic protein. Islet-1 expression is a reliable marker for pancreatic endocrine tumors and their metastases. Its expression is tightly regulated, and mutations in the ISL1 gene have been associated with congenital heart defects and developmental disorders. (Ref.: Sci Rep. 6:25928; Schick, E., et al. (2019). Sci Rep. 9(1):9358; Wang, Y., et al. (2019). Nat Commun. 10(1):4137; Bornhorst, D., et al. (2019). Nat Commun. 10(1):4113; Ma, Q., et al. (2020). Cell Rep. 31(3):107532; Lei, I., et al. (2020). Front Cell Dev Biol. 7:358; Vieira, J.R., et al. (2022). Neuron. 110(24):4074-4089; Carvelli, A., et al. (2022). EMBO J. 41(13):e108918.)

Physical form

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable