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Safety Information

MABN266

Sigma-Aldrich

Anti-SR-25 Antibody, clone 1F6.1

clone 1F6.1, from mouse

Synonym(s):

ADP-ribosylation factor-like protein 6-interacting protein 4, ARL-6-interacting protein 4, Aip-4, HSP-975, HSVI-binding protein, SR-15, SRp25, SR-25

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1F6.1, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_001002251

UniProt accession no.

Q66PJ3

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ARL6IP4(51329)

General description

Also known as ADP-ribosylation factor-like factor 6-interacting protein and ARL6IP4, the SR-25 protein is expressed in certain human organs and is relatively abundant in human testis and thymus. It is observed to mediate the Rac1 signaling pathway and acts as a splicing inhibitor of the Herpes simplex virus (HSVI); however, further research is needed to elucidate its mode of action.

Immunogen

GST-tagged recombinant protein corresponding to the C-terminus of human SR-25.

Application

Anti-SR-25 Antibody, clone 1F6.1 is a highly specific mouse monoclonal antibody, that targets SRp25 & has been tested in western blotting & ICC.
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected SR-25 in HeLa cells treated with Mimosine and Nocodazole.

Dylight® is a registered trademark of Thermo Fisher Scientific.
Research Category
Neuroscience
Research Sub Category
Signaling Neuroscience

Quality

Evaluated by Western Blotting in HeLa cells treated with Mimosine and Nocodazole.

Western Blotting Analysis: 1 µg/mL of this antibody detected SR-25 in HeLa cells treated with Mimosine and Nocodazole.

Target description

~32 kDa observed. Uniprot describes six isoforms between 22 kDa and 38 kDa

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

DyLight is a registered trademark of Pierce Biotechnology, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MABN266:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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