MABN2286

Anti-Rai Antibody, clone IF15

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: IF15-E2, monoclonal
• mol wt: calculated mol wt 64.06 kDa; observed mol wt ~58 kDa
• species reactivity: human, mouse
• packaging: antibody small pack of 100 μL
• technique(s): immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG
• UniProt accession no.: Q92529
• shipped in: dry ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

SHC-transforming protein 3 (UniProt: Q92529; also known as Neuronal Shc, N-Shc, Protein Rai, SHC-transforming protein C, Src homology 2 domain-containing-transforming protein C3, SH2 domain protein C3, Rai) is encoded by the SHC3 (also known as NSHC, SHCC) gene (Gene ID:53358) in human. Rai protein is mainly expressed in the brain and its higher expression is observed in cerebral cortex, frontal and temporal lobes, hippocampus, and occipital pole. Its expression is also reported in the enteric glial cells in the human gut. It serves as a signaling adapter and is involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons. Rai also serves as a physiological substrate of the receptor tyrosine kinase Ret and increases Ret-dependent survival via PI3 kinase/Akt pathway activation. It also potentiates MAP kinase signaling. Rai activation also plays a role in long-term potential and is neuroprotective against certain ischemia and oxidative stress. Evolutionarily it correlates well with vertebrate brain development. Its reduced levels are observed in brain of Alzheimer s disease subjects. Two isoforms of Rai have been described (p64 and p52) that are produced by alternative splicing. (Ref.: Triaca, V., et al. (2018). Aging. 10(1); 5-6; Sagi, O., et al. (2015). Ageing Res. Rev. 19; 34-42; Villanacci, V., et al. (2008). Neurogastroenterol. Motil. 20(3); 206-212).

Specificity

Clone IF15-E2 is a mouse monoclonal antibody that detects SHC-transforming protein 3/RAI. It targets an epitope within the CH1 domain from the C-terminal half.

Immunogen

Recombinant fragment corresponding to 164 amino acids CH1 domain of human SHC-transforming protein 3 (Rai).

Application

Quality Control Testing

Evaluated by Western Blotting in Human brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Rai in Human brain tissue lysate.

Tested Applications

Immunohistochemistry Applications: A representative lot detected Rai in Immunohistochemistry applications (Villanacci, V., et. al. (2008). Neurogastroenterol Motil. 20(3):206-12).

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Rai in U2OS cells.

Immunohistochemistry (Paraffin) Analysis: A 1:500 dilution from a representative lot detected Rai in human esophagus, stomach, and colon tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Rai, clone IF15, Cat. No. MABN2286, is a mouse monoclonal antibody that detects SHC-transforming protein 3 (Rai) and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Physical form

Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable