MABN15

Anti-Rhodopsin Antibody, clone 4D2

clone 4D2, from mouse

• Synonym(s): rhodopsin, rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant), retinitis pigmentosa 4, autosomal dominant, opsin 2, rod pigment
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 4D2, monoclonal
• species reactivity: rat, human, fish
• species reactivity (predicted by homology): mouse (based on 100% sequence homology)
• technique(s): ELISA: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_254276
• UniProt accession no.: P51489
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... RHO(6010) ; rat ... Rho(24717)

Description

General description

Rhodopsin is a multi-pass, G-protein-coupled membrane receptor protein belonging to the G-protein coupled receptor 1 family. Found within rod photoreceptor cells, rhodopsin is activated by light photons in order for the visual transduction cascade to initialize. Rhodopsin is necessary to the forming of images in low light and for the viability of photoreceptor cells after birth. Mutations in rhodopsin expression are the most widely known cause of the human disease retinitis pigmentosa (RP) resulting in gradual loss of vision. At present, there are over 100 known rhodopsin mutations.

Specificity

This antibody recognizes Rhodopsin at the N-terminus.

Immunogen

Epitope: N-terminus

Rat rod outer segments corresponding to rat Rhodopsin at the N-terminus.

Application

Anti-Rhodopsin Antibody, clone 4D2 is an antibody against Rhodopsin for use in IH, WB, IC, ELISA.

Research Category
Neuroscience

Research Sub Category
Sensory & PNS

Western Blot Analysis: A previous lot of this antibody detected Rhodopsin by WB in goldfish retina lysate, as reported by Knight K, et al.

Immunocytochemistry: A previous lot of this antibody detected Rhodopsin by ICC in goldfish retina tissue, as reported by Knight K, et al

Immunohistochemistry Analysis: 1:500 dilution of this antibody has been shown to detect Rhodopsin in human eye tissue.

ELISA: Specificity of this antibody, from a different lot, was validated in a competitive ELISA, as reported by Laird D, et al.

Quality

Evaluated by Immunohistochemistry in rat eye tissue.

Immunohistochemistry Analysis: 1:500 dilution of this antibody detected Rhodopsin in rat eye tissue.

Target description

Expected at ~ 39 kDa

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat eye tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable