MABF579

Anti-CD4 Antibody (human), APC-Cy7, clone RPA-T4

clone RPA-T4, from mouse

• Synonym(s): T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD antigen CD4
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.44

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: APC-Cy7
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: RPA-T4, monoclonal
• species reactivity: human
• packaging: pkg of 5 μL (0.5 μg/test)
• technique(s): flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• isotype: IgG1κ
• UniProt accession no.: P01730
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CD4(920)

Description

General description

The RPA-T4 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The RPA-T4 antibody is widely used as a phenotypic marker for human CD4 expression, and is cross-reactive with Chimpanzee CD4. This antibody recognizes a different epitope, and thus does not block binding of, the alternative Anti-Human CD4 antibody clone OKT4 (Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061-4065).

This mouse monoclonal antibody Anti-CD4 (human), APC-Cy7, clone RPA-T4 validated for use in IP, IHC & Flow Cytometry for the detection of CD4.

Quality

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 μg of this antibody detected CD4 in one million human peripheral blood lymphocytes.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg