MABF356

Anti-CD45RO (human) Antibody, FITC, clone UCHL1

clone UCHL1, from mouse, FITC conjugate

• Synonym(s): Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, CD45RO
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.44

Properties

• biological source: mouse
• conjugate: FITC conjugate
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: UCHL1, monoclonal
• species reactivity: human
• technique(s): flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2aκ
• UniProt accession no.: P08575
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PTPRC(5788)

Description

General description

Receptor-type tyrosine-protein phosphatase C, also known as Leukocyte common antigen (L-CA) or CD45 and encoded by the human gene PTPRC or CD45 is a transmembrane glycoprotein that belongs to the PTP family. PTPs are signaling molecules that regulate cellular process like cell growth, differentiation and mitotic cycle. CD45 has many isoforms that are distributed throughout the immune system according to cell type. These arise because of alternative splicing of RA, RB, and RC exons. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45RO is one of the isoforms of CD45 that facilitates T cell activation. It is also the shortest isform which lack the RA, RB, and RC exons. Mutations in CD45 have been associated with mulitple sclerosis and severe combined immunodeficiency.

Specificity

This antibody is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells.

Immunogen

Cultured T cells from an IL-2 dependent T-cell line (CA1) prepared from human peripheral blood.

Application

Anti-CD45RO (human) Antibody, FITC, clone UCHL1 is a highly specific mouse monoclonal antibody, that targets CD45RO & has been tested in Flow Cytometry, western blotting, IHC & IP.

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

Western Blotting Analysis: A representative lot detected human CD45RO in Western Blotting. (Ishii T, et al. 2001.)

Immunohistochemistry Analysis: A representative lot detected human CD45RO in Immunohistochemistry. (Yamada M, et al.1996.;Sakkas LI, et al. 1998.)

Immunoprecipitation Analysis: A representative lot detected human CD45RO in Immunoprecipitation. (Ponsford M, et al. 2001.)

Quality

The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.

Flow Cytometry Analysis: 20 μL of this antibody detected CD45RO in one million human peripheral blood lymphocytes.

Target description

147 kDa calculated

Physical form

Affinity

Purified mouse conjugate antibody in buffer containing PBS (pH 7.2) with up to 0.1% sodium azide and 0.2% (w/v) BSA.

Storage and Stability

The antibody solution should be stored undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable