MABF3184

Anti-RANTES/CCL5 Antibody, clone 15B5.6

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 15B5.6, monoclonal
• mol wt: calculated mol wt 9.99 kDa; observed mol wt ~8 kDa
• purified by: using protein G
• species reactivity: human
• packaging: antibody small pack of 100 μg
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG2aκ
• epitope sequence: Unknown
• Protein ID accession no.: NP_002976.2
• UniProt accession no.: P13501
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... CCL5(6352)

Description

General description

C-C motif chemokine 5 (UniProt: P13501; also known as EoCP, Eosinophil chemotactic cytokine, SIS-delta, Small-inducible cytokine A5, T cell-specific protein P228, TCP228, T-cell-specific protein RANTES, CCL5) is encoded by the CCL5 (also known as D17S136E, SCYA5) gene (Gene ID: 6352) in human. CCL5 is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to generate the mature protein (aa 24-91), which can further into RANTES (3-68) and RANTES (4-68). CCL5 serves as a chemoattractant for monocytes, memory T-helper cells, and eosinophils. It promotes immune cell recruitment to sites of inflammation. It also causes the release of histamine from basophils. It is involved in the activation of several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. The CCL5 activation of pancreatic cancer cells expressing CCR5 is reported to increase their invasive potential. CCL5 also functions as one of the natural ligands for the CCR5 and is reported to suppress in vitro replication of the R5 strains of HIV-1 that uses CCR5 as a coreceptor. The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a shown to be a potent inhibitor of HIV-1-infection. RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(3-68). (Ref.: Singh, SK., et al. (2018). Sci. Rep. 8; Article 1323; Lim, JK., et al. (2005). J. Leukoc Biol. 78(2); 442-452).

Specificity

Clone 15B5.6 is a mouse monoclonal antibody that detects RANTES/CCL5.

Immunogen

Full-length recombinant mature human RANTES/CCL5 (without the signal peptide).

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant human RANTES/CCL5.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant human RANTES/CCL5.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected RANTES/CCL5 in human spleen tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-RANTES/CCL5, clone 15B5.6, Cat. No. MABF3184, is a mouse monoclonal antibody that detects RANTES/CCL5 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG2a in PBS with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2