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MABE268

Sigma-Aldrich

Anti-Musashi-1 Antibody, clone 7B11.1

clone 7B11.1, from mouse

Synonym(s):

RNA-binding protein Musashi homolog 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

7B11.1, monoclonal

species reactivity

rat, mouse, human

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_002433

UniProt accession no.

O43347

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MSI1(4440)

General description

In mammals, the Musashi family is important for cell fate determination, playing roles in maintenance of the stem-cell state, cellular differentiation, nuclear signaling and tumorigenesis. Musashi-1 (also known as Msi1), is selectivley expressed in neural progenitor cells, including neural stem cells. Outside the nervous system, Musashi-1 is a selective marker for intestinal stem or early lineage cells. Musashi-1 interacts with the Notch pathway during asymmetric cell division by binding the 3′ UTR of Numb. Numb is prevented from repressing Notch signaling when Musashi-1 is present.

Immunogen

GST-tagged recombinant protein corresponding to human Musashi-1.

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Neural Stem Cells
Use Anti-Musashi-1 Antibody, clone 7B11.1 (Mouse Monoclonal Antibody) validated in WB, IHC to detect Musashi-1 also known as RNA-binding protein Musashi homolog 1.
Western Blot Analysis: A representative lot detected Musashi-1 in HEK293, NIH/3T3, and PC-12 cell lysate.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Musashi-1 in mouse cerebellum, normal mouse brain, and normal human pons tissue.

Quality

Evaluated by Western Blot in mouse E17 spinal cord tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Musashi-1 in 10 µg of mouse E17 spinal cord tissue lysate.

Target description

~39 kDa observed

Linkage

Replaces: 03-114; 04-1041

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse E17 spinal cord tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MABE268:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Thelma T Chiremba et al.
Molecular biology of the cell, 32(1), 28-44 (2020-11-12)
Evolutionarily conserved RNA-binding protein Musashi1 (Msi1) can regulate developmentally relevant genes. Here we report the generation and characterization of a mouse model that allows inducible Msi1 overexpression in a temporal and tissue-specific manner. We show that ubiquitous Msi1 induction in
Rebecca P McDonald et al.
Scientific reports, 8(1), 9648-9648 (2018-06-27)
Although lizards are often described as having robust neurogenic abilities, only a handful of the more than 6300 species have been explored. Here, we provide the first evidence of homeostatic neurogenesis in the leopard gecko (Eublepharis macularius). We focused our
Rebecca Pötschke et al.
Pediatric research, 87(4), 669-676 (2019-11-23)
Glioblastoma (GBM) is the most aggressive form of glioma in adults and children and is associated with very poor prognosis. Pediatric tumors are biologically distinct from adult GBM and differ in response to current GBM treatment protocols. Regarding pediatric GBM
E A B Gilbert et al.
The Journal of comparative neurology, 526(2), 285-309 (2017-10-06)
As for many lizards, the leopard gecko (Eublepharis macularius) can self-detach its tail to avoid predation and then regenerate a replacement. The replacement tail includes a regenerated spinal cord with a simple morphology: an ependymal layer surrounded by nerve tracts.
Takashi Shingu et al.
Nature genetics, 49(1), 75-86 (2016-11-15)
Stem cells, including cancer stem cells (CSCs), require niches to maintain stemness, yet it is unclear how CSCs maintain stemness in the suboptimal environment outside their niches during invasion. Postnatal co-deletion of Pten and Trp53 in mouse neural stem cells

Protocols

Neural Stem Cell Culture Protocols

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

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