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MABD10

Sigma-Aldrich

Anti-DMP1, NT Antibody, clone 9B6.3

clone 9B6.3, from mouse

Synonym(s):

Dentin matrix acidic phosphoprotein 1, DMP-1, Dentin matrix protein 1, AG1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9B6.3, monoclonal

species reactivity

rat

technique(s)

western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

rat ... Dmp1(25312)

General description

Dentin matrix acidic phosphoprotein is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene.

Specificity

This antibody recognizes the N-terminus of DMP1.

Immunogen

Epitope: N-terminus
N-terminus fragment of rat DMP1 isolated from long bone.

Application

Detect DMP1, N-terminus using this Anti-DMP1, N-terminus Antibody, clone 9B6.3 validated for use in Western Blotting.
Research Category
Stem Cell Research
Research Sub Category
Endothelial Stem Cells

Quality

Evaluated by Western Blot in rat dentin tissue extract.

Western Blot Analysis: 1 µg/mL of this antibody detected DMP1 in 10 µg of rat dentin tissue extract.

Target description

~65 kDa observed. Uniprot describes a molecular weight at ~53 kDa Uniprot describes that this protein is highly phosphorylated and highly glycosolated.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat dentin tissue extract

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MABD10:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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