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MABC48

Sigma-Aldrich

Anti-Anterior gradient protein 2 homolog Antibody, clone 7A10

clone 7A10, from mouse

Synonym(s):

anterior gradient homolog 2 (Xenopus laevis), anterior gradient 2 homolog, protein disulfide isomerase family A, member 17, Secreted cement gland protein XAG-2 homolog, anterior gradient protein 2 homolog, secreted cement gland homolog, anterior gradient

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

7A10, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_006399

UniProt accession no.

O95994

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... AGR2(10551)

General description

Anterior gradient protein 2 homolog (AGR2) (also known as secreted cement gland protein XAG-2 homolog) is thought to be a metastasis-inducing protein in breast cancer models and may be used as a means of detecting the outcome of hormonally treated patients with breast cancer. AGR2 may also play an important role in intestinal mucus production. Recent researched has also linked AGR2 to metastasis. Lower levels of AGR expression in prostate cancer may be highly predictive of the reoccurrence of disease even after a prostatectomy.

Specificity

Specific for Anterior gradient protein 2 homolog and does not detect Anterior gradient protein 3 homolog.

Immunogen

Linear peptide corresponding to a sequence unique to human Anterior gradient protein 2 homolog and not to human Anterior gradient protein 3 homolog.

Application

Anti-Anterior gradient protein 2 homolog Antibody, clone 7A10 is an antibody against Anterior gradient protein 2 homolog for use in WB & IC.
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC on primary carcinomas (Barraclough, D., et al. (2009). Am J Pathol. 175:1848–1857).
Research Category
Apoptosis & Cancer
Research Sub Category
Tumor Markers

Quality

Evaluated by Western Blot in MCF-7 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Anterior gradient protein 2 homolog in 10 µg of MCF-7 cell lysate.

Target description

~18 kDa observed

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
MCF-7 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MABC48:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Christopher Clarke et al.
Molecular and cellular biochemistry, 408(1-2), 245-252 (2015-07-15)
AGR2 is overexpressed in multiple cancers, particularly those arising from breast and prostate tissues, and higher levels of AGR2 are associated with earlier patient death. Although AGR2 is normally resident within the endoplasmic reticulum, the protein has been found in

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