MABC1168

Anti-LIMD2 Antibody, clone 18.30

clone 18.30, from mouse

• Synonym(s): LIM domain-containing protein
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 18.30, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_085053.1
• UniProt accession no.: Q9BT23
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... LIMD2(80774)

Description

General description

LIM domain-containing protein 2 (UniProt Q9BT23) is encoded by the LIMD2 (also known as SB143) gene (Gene ID 80774) in human. The LIM domain is organized as a tandem zinc-finger structure that functions as a modular protein-binding interface and LIM domain-containing proteins play diverse roles in gene expression, cyto-architecture, cell adhesion, cell motility, and signal transduction. Human LIM domain-only (LMO) proteins are involved in many normal developmental processes, as well as in the onset and progression of several cancers, including T-cell leukemia, breast cancer, and neuroblastoma. LIMD2 is a LMO protein that is highly expressed in papillary thyroid carcinoma (PTC) lymph node metastasis (LNM), but absent from the primary tumor or normal thyroid tissue in matched PTC patient samples. LIMD2 regulates cell motility and affects tumor progression via its role in the integrin-linked kinase (ILK) pathway. LIMD2 is reported to activate ILK activity via direct binding to ILK kinase domain near the active site, LIMD2-knockdown in cancer cells results in reduced cell migration. Likewise, epitopic LIMD2 expression promotes the migration of ILK-expressing fibroblasts, but not fibroblasts lacking ILK.

Specificity

Clone 18.30 detected endogenous LIMD2 in human (HEK293, U2OS, and TPC-1) cell lysates, as well as recombinant human LIMD2, but not LIMD1 or AJUBA protein (Peng, H., et al. (2014). Cancer Res. 74(5):1390-1403).

Immunogen

GST-tagged recombinant full-length human LIMD2.

Application

Anti-LIMD2 Antibody, clone 18.30 is an antibody against LIMD2 for use in Western Blotting, Immunohistochemistry (Paraffin), Immunocytochemistry.

Research Category
Apoptosis & Cancer

Research Sub Category
Adhesion (CAMs)

Western Blotting Analysis: 0.25 µg/mL from a representative lot detected LIMD2 in human U2OS osteosarcoma cell lysate.
Immunocytochemistry Analysis: A representative lot detected exogenously expressed LIMD2 protein constructs in formaldehyde-fixed, Triton X-100-permeabilized cells transfected with epitopically tagged LIMD2 (Peng, H., et al. (2014). Cancer Res. 74(5):1390-1403).
Immunohistochemistry Analysis: A representative lot detected LIMD2 immunoreactivity in formalin-fixed, paraffin-embedded papillary thyroid cancer and bladder tumor sections (Peng, H., et al. (2014). Cancer Res. 74(5):1390-1403).
Western Blotting Analysis: A representative lot detected endogenous LIMD2 in HEK293 and U2OS cell lysates, as well as epitopically tagged LIMD2, but not LIMD1 or AJUBA recombinant protein constructs (Peng, H., et al. (2014). Cancer Res. 74(5):1390-1403).

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected LIMD2 in human HEK293 kidney embryonic cell lysate.

Target description

~17 kDa observed. 14.07 kDa calculated. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable