MABC1138

Anti-HPCAL1 Antibody, clone 5A5

clone 5A5, from mouse

• Synonym(s): Hippocalcin-like protein 1, Calcium-binding protein BDR-1, HLP2, Visinin-like protein 3, VILIP-3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 5A5, monoclonal
• species reactivity: human
• packaging: antibody small pack of 25 μg
• technique(s): ELISA: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_602293.1
• UniProt accession no.: P37235
• target post-translational modification: unmodified
• Gene Information: human ... HPCAL1(3241)

Description

General description

Hippocalcin-like protein 1 (UniProt: P37235; also known as Calcium-binding protein BDR-1, HLP2, Visinin-like protein 3, VILIP-3) is encoded by the HPCAL1 (also known a BDR1) gene (Gene ID: 3241) in human. HPCAL1 is a lipid-anchor membrane protein of the visinin-like subfamily of neural calcium sensor and is involved in the calcium-dependent regulation of rhodopsin phosphorylation. It is structurally related to Ca2+-myristoyl switch proteins that contain four EF-hand motifs and a covalently attached N-terminal myristoyl group. HPCAL1 is also expressed in Purkinje cells of the cerebellum and hippocampus where it is believed to regulate synaptic plasticity relevant for learning and memory. Clone 5A5 can detect HPCAL1 in formalin-fixed 293 cells and in pancreatic cell lines. (Ref.: Chen, Y., et al. (2014). Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 33(1); 20-27; Li, C., et al. (2016). PLoS One 11(11); e0165921).

Specificity

Clone 5A5 specifically detects Hippocalcin-like protein 1 (HPCAL1) in human cells.

Immunogen

Native and denatured human HPCAL1.

Application

Detects Hippocalcin-like protein 1 using this mouse monoclonal Anti-HPCAL1, clone 5A5, Cat. No. MABC1138, tested for use in Western Blotting, Immunohistochemistry (Paraffin), ELISA and Immunocytochemistry.

Immunocytochemistry Analysis: A representative lot detected HPCAL1 in Immunocytochemistry applications (Chen, Y., et. al. (2014). Monoclon Antib Immunodiagn Immunother. 33(1):20-7).

ELISA Analysis: A representative lot detected HPCAL1 in ELISA applications (Chen, Y., et. al. (2014). Monoclon Antib Immunodiagn Immunother. 33(1):20-7).

Immunohistochemistry (Paraffin) Analysis: A 1:50-250 Dilution from a representative lot detected HPCAL1 in human kidney and human cerebellum tissue sections.

Research Category
Apoptosis & Cancer

Quality

Evaluated by Western Blotting in Panc-1 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected HPCAL1 in Panc-1 cell lysate.

Target description

~22 kDa observed; 22.13 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in PBS without azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable