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MAB995

Sigma-Aldrich

Anti-Bacterial Peptidoglycan Antibody, clone 3F6B3

ascites fluid, clone 3F6B3, Chemicon®

Synonym(s):

PG

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

300

antibody form

ascites fluid

antibody product type

primary antibodies

clone

3F6B3, monoclonal

species reactivity

bacteria

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable

isotype

IgG1

shipped in

wet ice

Specificity

Specific for the three-dimensional polymer complex structure of PG. The epitopes appear to consist of discontinuous glycan and/or amino acid residues, and have not been fully defined. In a competitive immunoassay format, several compounds were found to be ineffective as inhibitors: muramyldipeptide, N-acetylglucosamine (NAG), chitin (polymer of NAG) and acid-hydrolyzed chitin (NAG oligomers).

Immunogen

Insoluble PG obtained by TCA-heat and ethanol extraction of Streptococcus mutans BHT cells.

Application

May be used in solid-phase or competitive ELISA, immunodot-blot and immunocytochemistry formats. Although PG is a bacterial surface component, pre-treatments, such as treatment with strong acid (25mM HCl), for Gram-positive bacteria, or with a detergent, such as SDS or Triton-X (1% in PBS), for Gram-negative bacteria, may be necessary to expose the epitopes.

Optimal working dilutions must be determined by end user.
Research Category
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral
This Anti-Bacterial Peptidoglycan Antibody, clone 3F6B3 is validated for use in DB, ELISA, IC for the detection of Bacterial Peptidoglycan.

Physical form

Ascites fluid, no preservatives. Liquid.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MAB995:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Microbial communities reside in healthy tissues but are often disrupted during disease. Bacterial genomes and proteins are detected in brains from humans, nonhuman primates, rodents and other species in the absence of neurological disease. We investigated the composition and abundance
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