MAB5406B
Anti-GAD67 Antibody, clone 1G10.2, Biotin Conjugate
clone 1G10.2, from mouse, biotin conjugate
Synonym(s):
Glutamate decarboxylase 1, 67 kDa glutamic acid decarboxylase, GAD-67, Glutamate decarboxylase 67 kDa isoform
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
conjugate
biotin conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1G10.2, monoclonal
species reactivity
rat
species reactivity (predicted by homology)
human (based on 100% sequence homology), mouse (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... GAD1(2571)
General description
Gutamic acid decarboxylase (GAD; E.C. 4.1.1.15) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65 kDa and 67 kDa, 64% aa identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice (Sheikh, 1999). GAD65 is an amphiphilic, membrane-anchored protein (585 a.a.), encoded on human chromosome 10, and is responsible for vesicular GABA production. GAD67 is cytoplasmic (594 a.a.), encoded on chromosome 2, and seems to be responsible for significant cytoplasmic GABA production. GAD expression changes during neural development in rat spinal cord. GAD65 is expressed transiently in commissural axons around E13 but is down regulated the next day while GAD67 expression increases mostly in the somata of those neurons (Phelps, 1999). In mature rat pancreas, GAD65 and GAD67 appear to be differentially localized, GAD65 primarily in insulin-containing beta cells and GAD67 in glucagon-containing (A) cells (Li, 1995). GAD67 expression seems to be particularly plastic and can change in response to experimental manipulation (for example neuronal stimulation or transection) or disease progression and emergent disorders like schizophrenia (Volk, 2000). Colocalization of the two GAD isoforms also shows changes in GAD65/GAD67 distributions correlated with certain disease states such as IDDM and SMS.
Specificity
Reacts with GAD67. No detectable cross reactivity with GAD65 by Western blot on rat brain lysate.
Immunogen
Recombinant GAD67 protein
Application
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GAD67 in rat E18 primary cortex cells.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
This Anti-GAD Antibody, clone 1G10.2, Biotin Conjugate is validated for use in IHC for the detection of GAD67. See below for more information about this anti GAD antibody.
Quality
Evaluated by Immunohistochemistry in rat cortex tissue.
Immunohistochemistry Analysis: A 1:25 dilution of this antibody detected GAD67 in rat cortex tissue.
Immunohistochemistry Analysis: A 1:25 dilution of this antibody detected GAD67 in rat cortex tissue.
Target description
~67 kDa
Physical form
Protein A purified
Purified mouse monoclonal IgG2a conjugated to Biotin in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Storage and Stability
Maintain refrigerated at 2-8 °C in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
Rat cortex tissue
Rat cortex tissue
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
MAB5406B:
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Jose A Fernandez-Leon et al.
eLife, 10 (2021-12-17)
The recollection of environmental cues associated with threat or reward allows animals to select the most appropriate behavioral responses. Neurons in the prelimbic (PL) cortex respond to both threat- and reward-associated cues. However, it remains unknown whether PL regulates threat-avoidance
Jean-Christophe Delpech et al.
Science translational medicine, 13(611), eabe8455-eabe8455 (2021-09-16)
Abnormally phosphorylated tau, an early neuropathologic marker of Alzheimer’s disease (AD), first occurs in the brain’s entorhinal cortex layer II (ECII) and then spreads to the CA1 field of the hippocampus. Animal models of tau propagation aiming to recapitulate this
V Korzhova et al.
Communications biology, 4(1), 1368-1368 (2021-12-09)
Alzheimer's disease (AD) is associated with aberrant neuronal activity, which is believed to critically determine disease symptoms. How these activity alterations emerge, how stable they are over time, and whether cellular activity dynamics are affected by the amyloid plaque pathology
Zhi Ruan et al.
Brain : a journal of neurology, 144(1), 288-309 (2020-11-28)
Extracellular vesicles are highly transmissible and play critical roles in the propagation of tau pathology, although the underlying mechanism remains elusive. Here, for the first time, we comprehensively characterized the physicochemical structure and pathogenic function of human brain-derived extracellular vesicles
Andrijana Stanisavljević Ilić et al.
International journal of molecular sciences, 24(24) (2023-12-23)
Depression is linked to changes in GABAergic inhibitory neurons, especially parvalbumin (PV) interneurons, which are susceptible to redox dysregulation. Olanzapine (Olz) is an atypical antipsychotic whose mode of action remains unclear. We determined the effect of Olz on PV-positive (+)
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