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MAB5328

Sigma-Aldrich

Anti-RAGE Antibody, clone DD/A11 or A11

Chemicon®, from mouse

Synonym(s):

Receptor for Advanced Glycosylation End Products.

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

DD/A11, monoclonal
mAbA11, monoclonal

species reactivity

human, bovine, mouse

species reactivity (predicted by homology)

rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG2a

NCBI accession no.

NM_014226.1

UniProt accession no.

Q15109

shipped in

wet ice

target post-translational modification

glycosylation

Gene Information

human ... AGER(177)

General description

RAGE (Receptor for Advanced Glycosylation End Products) is a 35 kDa cell surface receptor that binds molecules modified by advanced glycation end products (AGEs), an irreversible non-enzymatic reaction occuring from the interaction of proteins and lipids with glucose. The increased expression of RAGE is associated with many disorders, such as diabetic vasculopathy, neuropathy, retinopathy and neuropathy, Alzheimer’s disease and immune/inflammatory reactions of the vessel walls. AGE/RAGE accumulates at sites of vascular disease in diabetes, but blockade of RAGE suppresses development of atherosclerosis and vascular disorders. RAGE binds to amyloid beta, which is overproduced in neurons and vessels in Alzheimer′s disease, and this interaction results in oxidative stress leading to neuronal degeneration.

Specificity

RAGE (Receptor for Advanced Glycosylated End Products). The antibody recognizes both natural and recombinant RAGE.

Immunogen

Recombinant truncated extracellular part of RAGE produced in E. coli.

Application

Anti-RAGE Antibody, clone DD/A11 or A11 is an antibody against RAGE for use in ELISA, IC, IH & WB.
Immunohistochemistry:
A previous lot of this antibody was shown to be reactive on paraffin embedded tissue sections.

Immunocytochemistry:
A previous lot was shown to work on cells fixed with ice cold acetone using a Cy3 conjugated secondary antibody.

ELISA:
A previous lot was shown to work on direct ELISA.

Immunoblotting:
Recognizes a band of ~48kDa in bovine lung extract.

Optimal working dilutions must be determined by end user.

Quality

Routinely evaluated by Western Blot on Mouse Brain lysates.

Western Blot:
1:500 dilution of this lot detected RAGE on 10 μg of Mouse Brain lysates.

Target description

48 kDa

Physical form

Format: Purified
Purified mouse monoclonal IgG2a in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl, and 0.1% sodium azide.

Analysis Note

Control
Lung tissue, mouse brain lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MAB5328:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Anna Kamynina et al.
Journal of cellular physiology, 236(9), 6496-6506 (2021-02-12)
The receptor for advanced glycation end products (RAGE) is a signal receptor first shown to be activated by advanced glycation end products, but also by a variety of signal molecules, including pathological advanced oxidation protein products and β-amyloid. However, most
Articular chondrocytes express the receptor for advanced glycation end products: Potential role in osteoarthritis.
Loeser, RF; Yammani, RR; Carlson, CS; Chen, H; Cole, A; Im, HJ; Bursch, LS; Yan, SD
Arthritis and Rheumatism null
Atsushi Fujiya et al.
Obesity (Silver Spring, Md.), 22(2), 371-379 (2013-06-28)
The S100 calcium binding protein B (S100B) implicated in brain inflammation acts via the receptor of advanced glycation end products (RAGE) and is also secreted from adipocytes. We investigated the role of S100B in the interaction between adipocytes and macrophages
Gareth D Healey et al.
Journal for immunotherapy of cancer, 7(1), 280-280 (2019-10-31)
The treatment of endometrial cancer (EC), the most common gynecological cancer, is currently hampered by the toxicity of current cytotoxic agents, meaning novel therapeutic approaches are urgently required. A cohort of 161 patients was evaluated for the expression of the
Philipp Ehlermann et al.
Cardiovascular diabetology, 5, 6-6 (2006-04-01)
Atherosclerosis is an inflammatory disease in which a perpetuated activation of NFkappaB via the RAGE (receptor for advanced glycation end products)-MAPK signalling pathway may play an important pathogenetic role. As recently S100 proteins have been identified as ligands of RAGE
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