MAB3404-C

Anti-Cytokeratin 18 Antibody, clone CK2 (Ascites Free)

clone CK2, 1 mg/mL, from mouse

• Synonym(s): Keratin, type I cytoskeletal 18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, CK-18, Keratin-18, K18
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: CK2, monoclonal
• species reactivity: human
• concentration: 1 mg/mL
• technique(s): immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_000215
• UniProt accession no.: P05783
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... KRT18(3875)

Description

General description

Cytokeratin 18, also known as Keratin, type I cytoskeletal 18, Cell proliferation-inducing gene 46 protein, CK-18, Keratin-18, or K18, and encoded by the gene KRT18/CYK18/PIG46, is involved in the uptake of thrombin-anti-thrombin complexes by hepatic cells. When the protein is phosphorylated it plays a role in filament reorganization. Cytokeratin-18 together keratin-8 and IL-6 are involved in skin barrier protection too, and cytokeratin-18 is often complexed with Cytokeratin 8. Cytokeratin-18 is expressed in colon, placenta, liver epithelia and many other tissues. Cytokeratin 18 in epithelial cells is cleaved by caspases during apoptosis to produce apoptotic specific CK-18 fragment that is the basis of many apoptosis detection assays.

Specificity

The antibody reacts with human cytokeratin 18 and is used for staining research samples of a wide variety of simple epithelia and simple glandular epithelia (e. g. intestinal, respiratory and urinary epithelia) and transitional epithelia (e. g. bladder), but stratified squamous epithelia are not stained (e. g. esophagus, epidermis).

Immunogen

Cytoskeletal preparation of HeLa cells expressing human Cytokeratin 18.

Application

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Cytokeratin 18 in human placenta tissue.

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Cyokeratin 18 in bladder tissue (Moll, R., et al. (1988). Am. J. Pathol. 132(1): 123-44.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Cyokeratin 18 in ductal carcinoma of the breast, basal cell epithelioma, squamous cell carcinoma of the tongue, squamous cell carcinoma of epiglottis, and squamous cell carcinoma of the esophagus (Debus, E., et al. (1984). Am. J. Pathol. 114(4): 121-30.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Cyokeratin 18 HeLa cell lysate (Debus, E., et al. (1984). Am. J. Pathol. 114(4): 121-30.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected Cyokeratin 18 in human epithelia tissue, uterus (cervic glands), submucosal glands of the oesophagus, and placental tissue (Debus, E., et al. (1984). Am. J. Pathol. 114(4): 121-30.).

Research Category
Cell Structure

Research Sub Category
Cytokeratins

This Anti-Cytokeratin 18 Antibody, clone CK2 (Ascites Free) is validated for use in IHC, western blotting & immunofluorescence for the detection of Cytokeratin 18.

Quality

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Cytokeratin 18 in human colorectal cancer tissue.

Target description

~48 kDa calculated

Linkage

Replaces: 04-586

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable