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MAB312-AF647

Sigma-Aldrich

Anti-A2B5 Antibody, clone A2B5-105 Antibody, Alexa Fluor™ 647 Conjugate

clone A2B5-105, from mouse, ALEXA FLUOR™ 647

Synonym(s):

F12A2B5, 105

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR™ 647

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

A2B5-105, monoclonal

species reactivity (predicted by homology)

mammals

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable

isotype

IgM

shipped in

ambient

target post-translational modification

unmodified

General description

A2B5 (also known as F12A2B5) is a cell surface ganglioside expressed on developing thymic epithelial cells, oligodendrocyte progenitors, and neuroendocrine cells. It is highly expressed in embryonic and neonatal tissue, but in the adult mammalian brain its expression is restricted mainly to areas that retain neurogenic potential, such as the subventricular zone (SVZ). Hence, A2B5 is considered as a marker for immature glial-committed precursors, which are permanently generated in the SVZ. Its expression is also considered as a marker for various cancers, including astrocytoma, glioma, pancreatic islet tumors, and squamous cell carcinoma. Explants from A2B5+ tumor cells display a typical progenitor morphology and clearly indicate their immature state. Tumors expressing A2B5 antigen exhibit higher rates of recurrence.

Specificity

This monoclonal antibody detects A2B5 in mammalian cells.

Immunogen

Embryonic chicken retinal cells.

Application

Anti-A2B5 Antibody, clone A2B5-105, Alexa Fluor™ 647 Conjugate Cat. No. MAB312-AF647, is a highly specific mouse monoclonal antibody that targets A2B5 and has been tested in Immunocytochemistry and Flow Cytometry.
Flow Cytometry Analysis: 1:400 dilution from a representative lot immunostained the surface of PC12 cells.

The unconjugated antibody (Cat. No. MAB312) is also available for flow cytometry, immunocytochemistry, immunofluorescence, and immunohistochemistry applications.

Quality

Evaluated by immunocytochemistry of PC12 cells.

Immunocytochemistry Analysis: A 1:100 Dilution of this antibody immunostained the surface of PC12 cells.

Physical form

Purified mouse IgM Alexa Fluor™ 647 conjugate in PBS with 1.5% BSA and 0.05% sodium azide.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MAB312-AF647:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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