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MAB1957

Sigma-Aldrich

Anti-Integrin β3 Antibody, clone 25E11

clone 25E11, Chemicon®, from mouse

Synonym(s):

CD61

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

25E11, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgM

NCBI accession no.

NM_000212.2

UniProt accession no.

P05106

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ITGB3(3690)

Specificity

Reacts with human integrin beta3 (GPIIIa, vitronectin receptor beta chain). The protein detectable is a complex of CD41 and CD61. The apparent molecular weight of the GPIIIa by SDS-PAGE is 105 kDa reduced and 90 kDa unreduced. Ligands are fibronectin, fibrinogen, von Willebrand factor, vitronectin and thrombospondin. Residues 237-248 of GPIIIa or CD61 are critical in adhesive protein binding. The monocytoid cell line U937 is highly positive.

(original paper claimed IgG2ak)

Immunogen

Normal blood mononuclear cells activated by mixed lymphocyte reaction and cultured for 10 days in medium containing IL-2.

Application

Immunoprecipitation

Immunocytochemistry (acetone fixation required)

Flow cytometry

Inhibits platelet aggregation, 10 μg/mL

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Integrins
This Anti-Integrin β3 Antibody, clone 25E11 is validated for use in FC, IP, IC, FUNC for the detection of Integrin β3.

Linkage

Replaces: 04-1060

Physical form

Format: Purified
In buffer of 0.02M phosphate, 0.25M sodium chloride, pH 7.6, 0.1% sodium azide.

Storage and Stability

Maintain between 2 and 8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MAB1957:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Diane Palmieri et al.
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Cyp1b1 mediates periostin regulation of trabecular meshwork development by suppression of oxidative stress.
Zhao, Y; Wang, S; Sorenson, CM; Teixeira, L; Dubielzig, RR; Peters, DM; Conway et al.
Molecular and cellular biology null
Francisco M Vega et al.
Open biology, 2(5), 120076-120076 (2012-06-23)
The Rho GTPase RhoB has been shown to affect cell migration, but how it does this is not clear. Here we show that cells depleted of RhoB by RNAi are rounded and have defects in Rac-mediated spreading and lamellipodium extension
Ujjwal K Rout
Neuroscience journal, 2013, 859257-859257 (2013-01-01)
The roles of integrin subunits and intracellular molecules in regulating the migration and neuritogenesis of neurons isolated from 16.5 gestation days rat fetal cortices were examined using in vitro assays. Results showed that laminin supported the migration of fetal cortical
S S Akimov et al.
The Journal of cell biology, 148(4), 825-838 (2000-02-23)
The protein cross-linking enzyme tissue transglutaminase binds in vitro with high affinity to fibronectin via its 42-kD gelatin-binding domain. Here we report that cell surface transglutaminase mediates adhesion and spreading of cells on the 42-kD fibronectin fragment, which lacks integrin-binding
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