MAB079-1
Anti-S-100 Protein Antibody, clone 15E2E2
clone 15E2E2, Chemicon®, from mouse
Synonym(s):
Calcium Binding Protein A1/B1
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
15E2E2, monoclonal
species reactivity
bovine, rat, human, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... S100A1(6271)
General description
S-100 protein is a member of the EF-hand calcium-binding protein superfamily that is widely distributed and conserved in the central nervous system of vertebrates. The protein exists in both heterodimeric and homodimeric forms. The two subunits of S-100 protein are the products of separate genes that are differentially expressed by various cells. The beta subunit is present in all S-100 positive cells and tumors. In contrast, the alpha subunit is detectable only in heart, skeletal muscle and brain. In addition to its calcium binding properties, S-100 protein has a high-affinity binding site for zinc and is involved in the regulation of protein phosphorylation in the brain. The protein apparently plays a role in cell differentiation and growth, cytoskeletal structure and function, and has been implicated in neuropathological diseases.
Specificity
This antibody recognizes ~22 kDa (non-reduced) or 11 kDa (reduced) of S100. It reacts with human, rat, mouse, and cow (bovine). Other species are not tested.
Immunogen
Epitope: Recognizes both alpha & beta S100
Purified bovine S-100 protein
Application
Anti-S-100 Protein Antibody, clone 15E2E2 is an antibody against S-100 Protein for use in IH, IH(P), IP & WB.
Immunoprecipitation:
A previous lot of this antibody was used in IP.
Visualization of cellular and subcellular localization of S-100 protein using immunohistochemical techniques.
Immunohistochemistry:
A 1:100-200 dilution of a previous lot was used for paraffin embedded tissue sections.
Optimal working dilutions must be determined by end user.
A previous lot of this antibody was used in IP.
Visualization of cellular and subcellular localization of S-100 protein using immunohistochemical techniques.
Immunohistochemistry:
A 1:100-200 dilution of a previous lot was used for paraffin embedded tissue sections.
Optimal working dilutions must be determined by end user.
Research Category
Metabolism
Metabolism
Research Sub Category
Muscle Physiology
Muscle Physiology
Quality
Evaluated by Western Blot on PC12 lysates
Western Blot Analysis:
1:1000 dilution of this antibody detected S100 on 10 µg of PC12 lysate.
Western Blot Analysis:
1:1000 dilution of this antibody detected S100 on 10 µg of PC12 lysate.
Physical form
Format: Purified
The mouse IgG is purified by protein A-affinity chromatography according to isotyping.
This affinity purified antibody is supplied in sterile phosphate buffered saline (pH 7.2) containing antibody stabilizer. Contains 0.01% sodium azide.
Storage and Stability
Stable for 1 year at 2-8ºC from date of receipt.
Analysis Note
Control
Positive Control Tissue: Melanoma
Positive Control Tissue: Melanoma
Other Notes
Concentration: 0.5 mg/mL
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
MAB079-1:
MAB079-1-KC:
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Lise Lyck, Jacob Jelsing, Pia S?ndergaard Jensen, Kate Lykke Lambertsen, Bente Pakkenberg et al.
Journal of Neuroscience Methods null
Steven Petratos et al.
Journal of neuropathology and experimental neurology, 62(4), 398-411 (2003-05-02)
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The Journal of pharmacology and experimental therapeutics, 354(2), 175-183 (2015-05-23)
The 3',5'-cAMP-adenosine pathway (3',5'-cAMP→5'-AMP→adenosine) and the 2',3'-cAMP-adenosine pathway (2',3'-cAMP→2'-AMP/3'-AMP→adenosine) are active in the brain. Oligodendrocytes participate in the brain 2',3'-cAMP-adenosine pathway via their robust expression of 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase; converts 2',3'-cAMP to 2'-AMP). Because Schwann cells also express CNPase
Daria Guseva et al.
Brain : a journal of neurology, 132(Pt 8), 2180-2195 (2009-06-23)
The adhesion molecule L1 is one of the few adhesion molecules known to be beneficial for repair processes in the adult central nervous system of vertebrates by promoting axonal growth and neuronal survival. In the peripheral nervous system, L1 is
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