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HTS136RTA

Sigma-Aldrich

Ready-to-Assay V1B Vasopressin Receptor Frozen Cells

Human

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About This Item

UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.76

product name

Ready-to-Assay V1B Vasopressin Receptor Frozen Cells,

biological source

human

Quality Level

100

manufacturer/tradename

Ready-to-Assay

technique(s)

calcium flux assay: suitable

NCBI accession no.

NM_000707

detection method

fluorometric

General description

Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Arginine vasopressin (AVP) is a 9 amino acid peptide that functions as an antidiuretic, vasoconstrictor and neurotransmitter. The three vasopressin receptors, V1A, V1B and V2, are GPCRs; V1A and V1B couple to Gq and calcium release, whereas V2 couples to Gs (Birnbaumer, 2000). The V1B receptor is expressed prominently in the anterior pituitary, where it mediates vasopressin-induced release of ACTH (Tanoue et al., 2004). A selective antagonist of V1B has recently been developed and shown to reduce depression, anxiety, and aggression in rodents (Blanchard et al, 2005; Griebel et al., 2002). Millipore′s cloned human V1B-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant V1B expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between V1B and its ligands.

Cell Line Description

  • GPCR Cell Lines
  • Host cells: Chem-1

Application

Human V1B GPCR frozen cells for Calcium Flux FLIPR Assays.

Biochem/physiol Actions

  • GPCR Class: A
  • Protein Target: V1B
  • Target Sub-Family: Vasopressin

Components

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
Fifty (50) mL of Media Component.

Disclaimer


This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

HTS136RTA:

Certificates of Analysis (COA)

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