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HTS064RTA

Sigma-Aldrich

Ready-to-Assay AT 1 Angiotensin Receptor Frozen Cells

Human

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About This Item

UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.76

product name

Ready-to-Assay AT 1 Angiotensin Receptor Frozen Cells,

biological source

human

Quality Level

100

manufacturer/tradename

Ready-to-Assay

technique(s)

calcium flux assay: suitable

NCBI accession no.

NM_000685

detection method

fluorometric

General description

Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Angiotensin II (Ang II), an octapeptide produced by cleavage of angiotensinogen by angiotensin-converting enzyme, plays a fundamental role in cardiovascular homeostasis. Two GPCRs, AT1 and AT2, mediate the effects of AngII, although AT1 is primarily responsible for the effects of Ang II on renal function and development, thirst, electrolyte and water balance, and arterial blood pressure. Binding of Ang II to AT1 activates both Gq and Gi (De Gasparo et al., 2000). Millipore′s cloned human AT1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant AT1 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at AT1.

Cell Line Description

  • GPCR Cell Lines
  • Host cells: Chem-1

Application

Human AT1 GPCR frozen cells for Calcium Flux FLIPR Assays.

Biochem/physiol Actions

  • GPCR Class: A
  • Protein Target: AT1
  • Target Sub-Family: Angiotensin

Components

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
Fifty (50) mL of Media Component.

Disclaimer


This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

HTS064RTA:

Certificates of Analysis (COA)

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